Mycobacterium phage AltPhacts
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Detailed Information for Phage AltPhacts
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMichael Murphy
Year Found2017
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.066 N, 118.448 W Map
Discovery NotesPhage AltPhacts was isolated from moist soil collected at a depth of 15cm on 4/11/17 by Michael Murphy. Sample was collected from a dumping area for liquids in a urban area. Air temp at time of collection was 20C. AltPhacts was isolated by enrichment plating at 30C (10X 7H9/glycerol medium, CaCl2, AD Supplement, M. smeg).

Characterization ws performed by Team Bac & Bougie (Megan Satyadi, Kalin Patel, Courtney Lee, Steve Yoon) and Team Google Genome (Eric He, Joel Forcheney, Olivia Yang, Kevin Hakimi).

Name of Individual who collected sample: Michael Murphy
Midi-Lysate (Archive) Titer: 1.75E10
Date of Midi-Lysate Titer: 5/10/17

Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Michelle Bina
Undergraduate Assistant: Rachel Zhu
Naming NotesWe named this phage "AltPhacts," a shortened version of Alternative Facts that is a reference to 2017 culture and politics.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 10, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)68056
Character of genome endsUnknown
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

Emiris (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque NotesPhage AltPhacts appears to be a temperate phage that demonstrated turbid, bullseye, and clear plaque morphologies on the same plate at both 30˚C and 35˚C, with a higher ratio of turbid/bullseye plaques at higher temperatures.
Average=2.5mm, Range=2-4mm
Normal Morphology: Clear, 2.5mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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AltPhacts_1
(terminase small subunit)

AltPhacts_2
(terminase large subunit)

AltPhacts_3

AltPhacts_4

AltPhacts_5

AltPhacts_6
(resolvase)

AltPhacts_7
(membrane protein)

AltPhacts_8
(portal protein)

AltPhacts_9
(capsid morphogenesis protein)

AltPhacts_10
(HNH endonuclease)

AltPhacts_11

AltPhacts_12
(major capsid protein)

AltPhacts_13

AltPhacts_14

AltPhacts_15
(membrane protein)

AltPhacts_16
(membrane protein)

AltPhacts_17
(membrane protein)

AltPhacts_18
(major tail protein)

AltPhacts_19

AltPhacts_20
(queuine tRNA-ribosyltransferase)

AltPhacts_21

AltPhacts_22

AltPhacts_23

AltPhacts_24

AltPhacts_25

AltPhacts_26

AltPhacts_27

AltPhacts_28
(tapemeasure protein)

AltPhacts_29
(minor tail protein)

AltPhacts_30
(minor tail protein)

AltPhacts_31
(minor tail protein)

AltPhacts_32
(minor tail protein)

AltPhacts_33
(minor tail protein)

AltPhacts_34

AltPhacts_35

AltPhacts_36

AltPhacts_37

AltPhacts_38

AltPhacts_39

AltPhacts_40

AltPhacts_41

AltPhacts_42

AltPhacts_43

AltPhacts_44
(membrane protein)

AltPhacts_45
(helix-turn-helix DNA binding domain protein)

AltPhacts_46
(helix-turn-helix DNA binding domain protein)

AltPhacts_47

AltPhacts_48
(lysin A)

AltPhacts_49
(lysin B)

AltPhacts_50

AltPhacts_51
(exonuclease)

AltPhacts_52

AltPhacts_53
(DNA helicase)

AltPhacts_54

AltPhacts_55

AltPhacts_56

AltPhacts_57
(DNA primase/polymerase)

AltPhacts_58

AltPhacts_59

AltPhacts_60
(DNA polymerase I)

AltPhacts_61

AltPhacts_62

AltPhacts_63
(membrane protein)

AltPhacts_64

AltPhacts_65

AltPhacts_66
(helix-turn-helix DNA binding domain protein)

AltPhacts_67

AltPhacts_68
(helix-turn-helix DNA binding domain protein)

AltPhacts_69

AltPhacts_70

AltPhacts_71

AltPhacts_72

AltPhacts_73
(helix-turn-helix DNA binding domain protein)

AltPhacts_74

AltPhacts_75

AltPhacts_76

AltPhacts_77

AltPhacts_78

AltPhacts_79
(membrane protein)

AltPhacts_80

AltPhacts_81

AltPhacts_82
(HNH endonuclease)

AltPhacts_83

AltPhacts_84

AltPhacts_85

AltPhacts_86

AltPhacts_87

AltPhacts_88

AltPhacts_89

AltPhacts_90

AltPhacts_91

AltPhacts_92

AltPhacts_93

AltPhacts_94

AltPhacts_95
(membrane protein)

AltPhacts_96

AltPhacts_97

AltPhacts_98

AltPhacts_99

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG962362
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# D7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload