Mycobacterium phage Boyle
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Detailed Information for Phage Boyle
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKaty Heslop and John Cole
Year Found2016
Location FoundGreensburg, PA United States
Finding InstitutionSeton Hill University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates40.309898 N, 79.557391 W Map
Discovery NotesBoyle was found under the stairs outside of Havey Hall at Seton Hill University. The climate was a warm September day, but did not reflect on the soil since the soil was under the stairs.
Naming NotesBoyle got his name in honor of the building he was purified in was named Joanne Woodyard Boyle Health Science Center.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1043
Genome length (bp)67491
Character of genome endsCircularly Permuted
GC Content69.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque NotesThe plaque morphology showed the sizes of clearing on the L-agar plate. The plate was completely clear, which means it is lytic phage.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Boyle_1
(helix-turn-helix DNA binding domain protein)

Boyle_2
(queuine tRNA-ribosyltransferase)

Boyle_3
(QueC-like queosine biosynthesis protein)

Boyle_4
(QueD-like queosine biosynthesis protein)

Boyle_5
(QueE-like queosine biosynthesis protein)

Boyle_6
(GTP cyclohydrolase)

Boyle_7
(terminase)

Boyle_8

Boyle_9
(resolvase)

Boyle_10

Boyle_11
(membrane protein)

Boyle_12
(portal protein)

Boyle_13
(capsid morphogenesis protein)

Boyle_14

Boyle_15
(major capsid protein)

Boyle_16

Boyle_17

Boyle_18
(membrane protein)

Boyle_19
(membrane protein)

Boyle_20

Boyle_21
(major tail protein)

Boyle_22

Boyle_23

Boyle_24
(head-to-tail connector protein)

Boyle_25

Boyle_26

Boyle_27
(tail assembly chaperone)

Boyle_28
(tail assembly chaperone)

Boyle_29
(tape measure protein)

Boyle_30
(minor tail protein)

Boyle_31
(minor tail protein)

Boyle_32
(minor tail protein)

Boyle_33
(minor tail protein)

Boyle_34

Boyle_35

Boyle_36

Boyle_37

Boyle_38

Boyle_39

Boyle_40

Boyle_41

Boyle_42

Boyle_43
(hydrolase)

Boyle_44
(helix-turn-helix DNA binding domain protein)

Boyle_45
(helix-turn-helix DNA binding domain protein)

Boyle_46

Boyle_47
(lysin A)

Boyle_48
(holin)

Boyle_49

Boyle_50

Boyle_51
(DNA helicase)

Boyle_52

Boyle_53

Boyle_54

Boyle_55
(dsDNA helicase)

Boyle_56

Boyle_57
(DNA polymerase I)

Boyle_58

Boyle_59

Boyle_60

Boyle_61

Boyle_62

Boyle_63

Boyle_64
(ribbon helix-helix DNA binding domain protein)

Boyle_65

Boyle_66
(helix-turn-helix DNA binding domain protein)

Boyle_67

Boyle_68

Boyle_69
(HNH endonuclease)

Boyle_70
(HNH endonuclease)

Boyle_71

Boyle_72
(HicA-like toxin)

Boyle_73

Boyle_74

Boyle_75

Boyle_76

Boyle_77

Boyle_78

Boyle_79

Boyle_80

Boyle_81
(helix-turn-helix DNA binding domain protein)

Boyle_82

Boyle_83

Boyle_84

Boyle_85

Boyle_86

Boyle_87
(membrane protein)

Boyle_88

Boyle_89

Boyle_90

Boyle_91

Boyle_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH051249
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 33
Pitt Freezer Box Grid# E12
Available Files
GenBank File for PhameratorDownload