Mycobacterium phage Phergie
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Phergie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDivya Balchander & Kristofer Apellido
Year Found2015
Location FoundPhiladelphia, PA United States
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.95681 N, 75.18959 W Map
Discovery NotesCollected in the Drexel University College of Computing and Informatics Garden, by the cascading fountain.

Conditions during Collection:
Approximately 72 °F with little to mild humidity
sample collected 2 inches below the surface from moist soil, surrounded by foliage.

Naming NotesOur phage is inspired by the artist Fergie.

"If you ain't got no plaques take yo' broke plate home
You say: If you ain't got no plaques take yo'broke plate home
S-E-A-P-H-A-G-E-S, yeah S-E-A-P-H-A-G-E-S

We flying the first class
Up in the sky
Poppin' M.smeg.
Livin' my life
In the fast lane
And I won't change
For the glamorous, oh infecting bacteria, bacteria."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1276
Genome length (bp)68716
Character of genome endsCircularly Permuted
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

Click to View

Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JackyB (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Annotation StatusIn GenBank
Plaque NotesPlaque shape noted as circular, size is 3.5mm on average , and turbidity has been consistently slightly turbid.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Phergie_1
(terminase, small subunit)

Phergie_2
(terminase, large subunit)

Phergie_3

Phergie_4

Phergie_5

Phergie_6
(resolvase. RuvC-like)

Phergie_7

Phergie_8
(portal protein)

Phergie_9
(capsid morphogenesis protein)

Phergie_10

Phergie_11
(major capsid protein)

Phergie_12

Phergie_13

Phergie_14

Phergie_15

Phergie_16

Phergie_17
(major tail protein)

Phergie_18

Phergie_19
(queuine tRNA-ribosyltranferase)

Phergie_20

Phergie_21

Phergie_22

Phergie_23

Phergie_24

Phergie_25

Phergie_26

Phergie_27
(tape measure protein)

Phergie_28
(minor tail protein)

Phergie_29
(minor tail protein)

Phergie_30
(minor tail protein)

Phergie_31
(minor tail protein)

Phergie_32
(minor tail protein)

Phergie_33

Phergie_34

Phergie_35

Phergie_36

Phergie_37

Phergie_38

Phergie_39

Phergie_40

Phergie_41

Phergie_42

Phergie_43

Phergie_44

Phergie_45
(helix-turn-helix DNA binding domain)

Phergie_46
(helix-turn-helix DNA binding domain)

Phergie_47
(lysin A, peptidase domain)

Phergie_48
(lysin A, amidase domain)

Phergie_49
(Lysin B)

Phergie_50

Phergie_51

Phergie_52
(DNA helicase)

Phergie_53

Phergie_54

Phergie_55

Phergie_56

Phergie_57

Phergie_58

Phergie_59
(DNA primase/polymerase)

Phergie_60

Phergie_61

Phergie_62
(DNA polymerase I)

Phergie_63

Phergie_64

Phergie_65

Phergie_66

Phergie_67

Phergie_68

Phergie_69

Phergie_70
(RNase E endoribonuclease)

Phergie_71

Phergie_72

Phergie_73

Phergie_74

Phergie_75

Phergie_76

Phergie_77

Phergie_78

Phergie_79

Phergie_80

Phergie_81

Phergie_82

Phergie_83

Phergie_84
(HNH endonuclease)

Phergie_85

Phergie_86

Phergie_87

Phergie_88

Phergie_89

Phergie_90

Phergie_91

Phergie_92

Phergie_93

Phergie_94

Phergie_95

Phergie_96

Phergie_97

Phergie_98

Phergie_99

Phergie_100

Phergie_101

Phergie_102

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG757165
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload