Mycobacterium phage Vincenzo
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Detailed Information for Phage Vincenzo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGabrielle Gentile
Year Found2014
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.442252 N, 79.952284 W Map
Discovery NotesI extracted my soil from a forest trail in Schenley Park on Wednesday, September 03, 2014 at 11:15 AM. The selected region was shaded by numerous trees. The soil was dark brown and moist and taken from the surface of of the ground. There was much plant growth surrounding the soil including grass and green plants. The weather conditions were warm, yet cool under the shade.
Naming NotesI chose to name my phage Vincenzo because it is an unusual name to give a virus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage194
Genome length (bp)72139
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB4
Other Cluster Members

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Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

Cannibal (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulu100 (B1)

Lulumae (B1)

Mag7 (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Megatron (B1)

Mesh1 (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

RedMaple (B1)

Rfink25 (B1)

Riggan (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Rstan (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Kamiyu (B3)

Littleengine (B3)

Mortcellus (B3)

NOZO (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RomaT (B3)

SassBadger (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Plaque NotesA 10^4 dilution of phage Vincenzo was used to infect Mycobacterium smegmatis and plated on 7H10 medium. Plates were incubated at 30ºC for 48 hours. Vincenzo typically forms clear plaques with an average size <1mm, but some variation in plaque size (up to 1mm) is frequently observed.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Vincenzo_1

Vincenzo_2
(carboxylate deaminase)

Vincenzo_3

Vincenzo_4
(terminase small subunit)

Vincenzo_5
(terminase large subunit/(homing endonuclease in intein))

Vincenzo_6

Vincenzo_7

Vincenzo_8
(RuvC)

Vincenzo_9

Vincenzo_10
(portal)

Vincenzo_11
(capsid morphogenesis protein)

Vincenzo_12

Vincenzo_13
(major capsid protein)

Vincenzo_14

Vincenzo_15

Vincenzo_16

Vincenzo_17

Vincenzo_18

Vincenzo_19
(major tail subunit)

Vincenzo_20

Vincenzo_21

Vincenzo_22

Vincenzo_23

Vincenzo_24
(tail assembly chaperone)

Vincenzo_25
(tail assembly chaperone)

Vincenzo_26
(tapemeasure protein)

Vincenzo_27
(minor tail protein)

Vincenzo_28
(minor tail protein)

Vincenzo_29
(minor tail protein)

Vincenzo_30
(minor tail protein)

Vincenzo_31
(minor tail protein)

Vincenzo_32

Vincenzo_33

Vincenzo_34

Vincenzo_35

Vincenzo_36

Vincenzo_37

Vincenzo_38

Vincenzo_39

Vincenzo_40

Vincenzo_41
(AlpA-like)

Vincenzo_42
(HTH DNA binding protein)

Vincenzo_43

Vincenzo_44
(LysA)

Vincenzo_45
(LysB)

Vincenzo_46

Vincenzo_47

Vincenzo_48

Vincenzo_49
(DNA helicase)

Vincenzo_50

Vincenzo_51

Vincenzo_52

Vincenzo_53
(primase/helicase)

Vincenzo_54

Vincenzo_55

Vincenzo_56
(DNA polymerase I)

Vincenzo_57

Vincenzo_58

Vincenzo_59

Vincenzo_60

Vincenzo_61

Vincenzo_62

Vincenzo_63

Vincenzo_64

Vincenzo_65

Vincenzo_66

Vincenzo_67

Vincenzo_68

Vincenzo_69

Vincenzo_70
(RNase E)

Vincenzo_71

Vincenzo_72

Vincenzo_73

Vincenzo_74

Vincenzo_75

Vincenzo_76

Vincenzo_77

Vincenzo_78
(HNH endonuclease)

Vincenzo_79

Vincenzo_80

Vincenzo_81

Vincenzo_82

Vincenzo_83

Vincenzo_84

Vincenzo_85

Vincenzo_86

Vincenzo_87

Vincenzo_88

Vincenzo_89

Vincenzo_90

Vincenzo_91

Vincenzo_92

Vincenzo_93

Vincenzo_94

Vincenzo_95

Vincenzo_96

Vincenzo_97

Vincenzo_98

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKR080194
Refseq NumberNone yet
Published in PaperPope WH et al 2015, Genome Announcements
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload