Mycobacterium phage CharlieGBrown
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Detailed Information for Phage CharlieGBrown
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJenny Samiec and Jackie Etson
Year Found2014
Location FoundColumbus, OH United States
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature25°C
GPS Coordinates40.0013 N, 83.0146 W Map
Discovery NotesPhage isolated in a biology lab at The Ohio State University. Possibly a temperate phage with a mostly lytic lifestyle. EcoRI made one cut on the phage's DNA during the RE digest.
Naming NotesWe decided to name it Charliegbrown because Christmas is coming!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1868
Genome length (bp)68604
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Other Cluster Members

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Alis (None)

Azarath (None)

BigMau (None)

BlaqueIvy (None)

Cally (None)

Camden (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

Skye (None)

Themis (None)

ABU (B1)

Alex (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

Cannibal (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulu100 (B1)

Lulumae (B1)

Mag7 (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Megatron (B1)

Mesh1 (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

RedMaple (B1)

Rfink25 (B1)

Riggan (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Rstan (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Kamiyu (B3)

Littleengine (B3)

Mortcellus (B3)

NOZO (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RomaT (B3)

SassBadger (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Plaque NotesPlaques were small, round, about 2mm in diameter. Clear most of the time.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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CharlieGBrown_1
(Terminase, small subunit)

CharlieGBrown_2
(Terminase, large subunit)

CharlieGBrown_3

CharlieGBrown_4

CharlieGBrown_5

CharlieGBrown_6
(resolvase, RuvC-like)

CharlieGBrown_7

CharlieGBrown_8
(Portal Protein)

CharlieGBrown_9
(Portal Protein)

CharlieGBrown_10

CharlieGBrown_11
(Major capsid protein)

CharlieGBrown_12

CharlieGBrown_13

CharlieGBrown_14

CharlieGBrown_15

CharlieGBrown_16

CharlieGBrown_17
(Major tail protein)

CharlieGBrown_18

CharlieGBrown_19
(Queuine tRNA-ribosyltransferase)

CharlieGBrown_20

CharlieGBrown_21

CharlieGBrown_22

CharlieGBrown_23

CharlieGBrown_24

CharlieGBrown_25

CharlieGBrown_26

CharlieGBrown_27
(Tape measure Protein)

CharlieGBrown_28
(Minor Tail protein)

CharlieGBrown_29
(Minor Tail protein)

CharlieGBrown_30
(Minor Tail protein)

CharlieGBrown_31
(Minor Tail protein)

CharlieGBrown_32
(Minor Tail protein)

CharlieGBrown_33

CharlieGBrown_34

CharlieGBrown_35

CharlieGBrown_36

CharlieGBrown_37

CharlieGBrown_38

CharlieGBrown_39

CharlieGBrown_40

CharlieGBrown_41

CharlieGBrown_42

CharlieGBrown_43

CharlieGBrown_44
(helix-turn-helix DNA binding domain)

CharlieGBrown_45
(helix-turn-helix DNA Binding Domain)

CharlieGBrown_46

CharlieGBrown_47
(lysin A)

CharlieGBrown_48
(lysin B)

CharlieGBrown_49

CharlieGBrown_50

CharlieGBrown_51
(DNA Helicase)

CharlieGBrown_52

CharlieGBrown_53

CharlieGBrown_54

CharlieGBrown_55

CharlieGBrown_56

CharlieGBrown_57

CharlieGBrown_58
(DNA primase/helicase)

CharlieGBrown_59

CharlieGBrown_60

CharlieGBrown_61
(DNA Polymerase)

CharlieGBrown_62

CharlieGBrown_63

CharlieGBrown_64

CharlieGBrown_65

CharlieGBrown_66

CharlieGBrown_67

CharlieGBrown_68
(RNaseE)

CharlieGBrown_69

CharlieGBrown_70

CharlieGBrown_71
(phospholipase (PLA2-like))

CharlieGBrown_72

CharlieGBrown_73
(helix-turn-helix DNA Binding domain)

CharlieGBrown_74

CharlieGBrown_75

CharlieGBrown_76

CharlieGBrown_77

CharlieGBrown_78

CharlieGBrown_79

CharlieGBrown_80

CharlieGBrown_81

CharlieGBrown_82
(HNH Endonuclease)

CharlieGBrown_83

CharlieGBrown_84

CharlieGBrown_85

CharlieGBrown_86

CharlieGBrown_87

CharlieGBrown_88

CharlieGBrown_89

CharlieGBrown_90

CharlieGBrown_91

CharlieGBrown_92

CharlieGBrown_93

CharlieGBrown_94

CharlieGBrown_95

CharlieGBrown_96

CharlieGBrown_97

CharlieGBrown_98

CharlieGBrown_99

CharlieGBrown_100

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX576647
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload