Mycobacterium phage ItsyBitsy1
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Detailed Information for Phage ItsyBitsy1
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesDangGina
Found ByKatie Mustin, Benita Platas, Stacie Williams
Year Found2016
Location FoundWylie, TX United States
Finding InstitutionCollin College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates32.978679 N, 96.52515 W Map
Discovery NotesThe phage was found in a residential neighborhood in a flower bed near a home.
When obtained there was a large and thick layer of mulch at the top of the soil. The soil was loose and dry the temperature was 76 degrees F and a cloudy morning (7:52 a.m.)
Naming NotesNamed after a saying.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3332
Genome length (bp)67570
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

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Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

LilMcDreamy (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

TommyBoy (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

Beaglebox (B1)

BengaliBalla92 (B1)

Beni05 (B1)

Bishoperium (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

CamL (B1)

Cannibal (B1)

Carthage (B1)

Ceraveeno (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

Doc (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

Emiris (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Eugenia (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

Frankicide (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Gophee (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kahve (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

Lauryn (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Llorona (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Matalotodo (B1)

Mecca (B1)

Megatron (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinheadLarry (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

QueenBeane (B1)

RedMaple (B1)

Riggan (B1)

Robyn (B1)

Rolex (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17a (B2)

Tinciduntsolum (B2)

Tres (B2)

West99 (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Giraffey (B3)

Gulsi (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Littleengine (B3)

Marley1013 (B3)

Mortcellus (B3)

Morty007 (B3)

Nozo (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Tydolla (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

Hangman (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Waleliano (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Larry28 (B7)

Saguaro (B7)

Tapir (B7)

Thonko (B8)

Annotation StatusIn GenBank
Plaque NotesThe size of the plaques are very tiny, they ranged between 0.5 mm-1.5 mm. They also grew clear and in a circular shape.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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ItsyBitsy1_1
(helix-turn-helix DNA binding domain protein)

ItsyBitsy1_2
(queuine tRNA-ribosyltransferase)

ItsyBitsy1_3
(QueC-like queosine biosynthesis protein)

ItsyBitsy1_4
(QueD-like queosine biosynthesis protein)

ItsyBitsy1_5
(QueE-like queosine biosynthesis protein)

ItsyBitsy1_6
(GTP cyclohydrolase I)

ItsyBitsy1_7
(terminase)

ItsyBitsy1_8

ItsyBitsy1_9
(resolvase)

ItsyBitsy1_10

ItsyBitsy1_11

ItsyBitsy1_12
(portal protein)

ItsyBitsy1_13
(capsid morphogenesis protein)

ItsyBitsy1_14

ItsyBitsy1_15
(major capsid protein)

ItsyBitsy1_16

ItsyBitsy1_17

ItsyBitsy1_18

ItsyBitsy1_19

ItsyBitsy1_20

ItsyBitsy1_21
(major tail protein)

ItsyBitsy1_22

ItsyBitsy1_23

ItsyBitsy1_24

ItsyBitsy1_25

ItsyBitsy1_26

ItsyBitsy1_27
(tail assembly chaperone)

ItsyBitsy1_28
(tail assembly chaperone)

ItsyBitsy1_29
(tape measure protein)

ItsyBitsy1_30
(minor tail protein)

ItsyBitsy1_31
(minor tail protein)

ItsyBitsy1_32
(minor tail protein)

ItsyBitsy1_33
(minor tail protein)

ItsyBitsy1_34

ItsyBitsy1_35

ItsyBitsy1_36

ItsyBitsy1_37

ItsyBitsy1_38

ItsyBitsy1_39

ItsyBitsy1_40

ItsyBitsy1_41

ItsyBitsy1_42

ItsyBitsy1_43
(hydrolase)

ItsyBitsy1_44
(helix-turn-helix DNA binding domain protein)

ItsyBitsy1_45
(helix-turn-helix DNA binding domain protein)

ItsyBitsy1_46

ItsyBitsy1_47
(lysin A)

ItsyBitsy1_48
(holin)

ItsyBitsy1_49

ItsyBitsy1_50

ItsyBitsy1_51
(DNA helicase)

ItsyBitsy1_52

ItsyBitsy1_53

ItsyBitsy1_54

ItsyBitsy1_55
(DNA primase/polymerase)

ItsyBitsy1_56

ItsyBitsy1_57
(DNA polymerase I)

ItsyBitsy1_58

ItsyBitsy1_59

ItsyBitsy1_60

ItsyBitsy1_61

ItsyBitsy1_62

ItsyBitsy1_63
(ribbon-helix-helix DNA binding protein)

ItsyBitsy1_64

ItsyBitsy1_65
(helix-turn-helix DNA binding domain protein)

ItsyBitsy1_66

ItsyBitsy1_67

ItsyBitsy1_68
(helix-turn-helix DNA binding domain protein)

ItsyBitsy1_69
(HNH endonuclease)

ItsyBitsy1_70
(HicA-like toxin)

ItsyBitsy1_71

ItsyBitsy1_72

ItsyBitsy1_73

ItsyBitsy1_74

ItsyBitsy1_75

ItsyBitsy1_76

ItsyBitsy1_77

ItsyBitsy1_78

ItsyBitsy1_79

ItsyBitsy1_80

ItsyBitsy1_81

ItsyBitsy1_82

ItsyBitsy1_83

ItsyBitsy1_84

ItsyBitsy1_85

ItsyBitsy1_86

ItsyBitsy1_87

ItsyBitsy1_88

ItsyBitsy1_89

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG812491
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.4x10^10
Pitt Freezer Box# 32
Pitt Freezer Box Grid# G1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload