| Detailed Information for Phage Horchata |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Elizabet Cabrera |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.0722 N, 118.4441 W Map |
| Discovery Notes | Phage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Phage Hunters: Elizabet Cabrera, Eduardo Silva. Eric Zhou, Pawit Tangmatitam. Annotation quality checked by Andrew Lund. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).
Sample description: Soil collected by Elizabet Cabrera on April 2, 2014.Extremely moist sample acquired from sloped hill facing South in garden on Gayley Ave. Little to no foot traffic and plenty of surrounding trees and plants. Depth from which sample was taken (in inches or cm): 1 cm |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 456 |
| Genome length (bp) | 68171 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Plaques display bulls-eye morphology but genome does not contain an integrase gene. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Characteristic bulls-eye morphology with a size range of 3 to 5 mm during the 4 purification plaque assays. Average plaque diameter was 4.22 mm. Plaques develop from extremely turbid to bulls-eye morphologies with clear centers and turbid halos with repeated culturing. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919507 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
D6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
