Mycobacterium phage Horchata
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Detailed Information for Phage Horchata
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByElizabet Cabrera
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.0722 N, 118.4441 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Phage Hunters: Elizabet Cabrera, Eduardo Silva. Eric Zhou, Pawit Tangmatitam. Annotation quality checked by Andrew Lund. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Soil collected by Elizabet Cabrera on April 2, 2014.Extremely moist sample acquired from sloped hill facing South in garden on Gayley Ave. Little to no foot traffic and plenty of surrounding trees and plants. Depth from which sample was taken (in inches or cm): 1 cm
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage456
Genome length (bp)68171
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Other Cluster Members

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Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

Cannibal (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulu100 (B1)

Lulumae (B1)

Mag7 (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Megatron (B1)

Mesh1 (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

RedMaple (B1)

Rfink25 (B1)

Riggan (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Rstan (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Kamiyu (B3)

Littleengine (B3)

Mortcellus (B3)

NOZO (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RomaT (B3)

SassBadger (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Plaque NotesCharacteristic bulls-eye morphology with a size range of 3 to 5 mm during the 4 purification plaque assays. Average plaque diameter was 4.22 mm. Plaques develop from extremely turbid to bulls-eye morphologies with clear centers and turbid halos with repeated culturing.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes96
Has been Phamerated?Yes
Gene List

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Horchata_1
(terminase, small subunit)

Horchata_2
(terminase, large subunit)

Horchata_3

Horchata_4

Horchata_5

Horchata_6
(resolvase, RuvC-like)

Horchata_7

Horchata_8
(portal protein)

Horchata_9
(capsid morphogenesis protein)

Horchata_10
(HNH endonuclease)

Horchata_11

Horchata_12
(major capsid protein)

Horchata_13

Horchata_14

Horchata_15

Horchata_16

Horchata_17

Horchata_18
(major tail protein)

Horchata_19

Horchata_20
(quenine tRNA-ribosyltransferase)

Horchata_21

Horchata_22

Horchata_23

Horchata_24

Horchata_25

Horchata_26

Horchata_27

Horchata_28
(tape measure protein)

Horchata_29
(minor tail protein)

Horchata_30
(minor tail protein)

Horchata_31
(minor tail protein)

Horchata_32
(minor tail protein)

Horchata_33
(minor tail protein)

Horchata_34

Horchata_35

Horchata_36

Horchata_37

Horchata_38

Horchata_39

Horchata_40

Horchata_41

Horchata_42

Horchata_43

Horchata_44

Horchata_45
(winged helix DNA binding domain)

Horchata_46
(helix-turn-helix DNA binding domain)

Horchata_47

Horchata_48
(lysin A)

Horchata_49
(lysin B)

Horchata_50

Horchata_51

Horchata_52
(DNA helicase)

Horchata_53

Horchata_54

Horchata_55

Horchata_56

Horchata_57
(DNA primase/polymerase)

Horchata_58

Horchata_59
(DNA polymerase I)

Horchata_60

Horchata_61

Horchata_62

Horchata_63

Horchata_64

Horchata_65

Horchata_66

Horchata_67
(Rnase E endoribonuclease)

Horchata_68

Horchata_69

Horchata_70

Horchata_71

Horchata_72
(helix-turn-helix DNA binding domain)

Horchata_73

Horchata_74

Horchata_75

Horchata_76

Horchata_77

Horchata_78

Horchata_79

Horchata_80

Horchata_81
(HNH endonuclease)

Horchata_82

Horchata_83

Horchata_84

Horchata_85

Horchata_86

Horchata_87

Horchata_88

Horchata_89

Horchata_90

Horchata_91

Horchata_92

Horchata_93

Horchata_94

Horchata_95

Horchata_96

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919507
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload