Mycobacterium phage LemonSlice
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Detailed Information for Phage LemonSlice
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByYing-Chiang Jeffrey Lee and Claire Brownstone
Year Found2011
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.643844 N, 90.315294 W Map
Discovery NotesMoisture: Dry
Depth: 3cm
Air Temperature: 66º F
Features: In an urban environment, next to a brick and cement path frequented by foot traffic. Decaying vegetation nearby. In a shadow during parts of the day due to vegetation cover nearby.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 10, 2013
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage180
Sanger Finishing Reactions2
Genome length (bp)68324
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Other Cluster Members

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Alis (None)

Azarath (None)

BigMau (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

ABU (B1)

Alex (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

Cannibal (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulu100 (B1)

Lulumae (B1)

Mag7 (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Megatron (B1)

Mesh1 (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

RedMaple (B1)

Rfink25 (B1)

Riggan (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Rstan (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Kamiyu (B3)

Littleengine (B3)

Mortcellus (B3)

NOZO (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RomaT (B3)

SassBadger (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Plaque NotesPlaque shape relatively consistent. Plaques were all circular with varying sizes due to different dilutions.
Clear, translucent plaque.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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LemonSlice_1
(terminase, small subunit)

LemonSlice_2
(terminase, large subunit)

LemonSlice_3

LemonSlice_4

LemonSlice_5

LemonSlice_6
(resolvase, RuvC-like)

LemonSlice_7

LemonSlice_8
(Portal Protein)

LemonSlice_9
(Capsid Morphogenisis Protein)

LemonSlice_10

LemonSlice_11

LemonSlice_12
(major capsid protein)

LemonSlice_13

LemonSlice_14

LemonSlice_15

LemonSlice_16

LemonSlice_17

LemonSlice_18
(major tail protein)

LemonSlice_19

LemonSlice_20
(queuine tRNA-ribosyltransferase)

LemonSlice_21

LemonSlice_22

LemonSlice_23

LemonSlice_24

LemonSlice_25
(minor capsid protein)

LemonSlice_26

LemonSlice_27

LemonSlice_28
(tape measure protein)

LemonSlice_29
(minor tail protein)

LemonSlice_30
(minor tail protein)

LemonSlice_31
(minor tail protein)

LemonSlice_32
(minor tail protein)

LemonSlice_33
(minor tail protein)

LemonSlice_34

LemonSlice_35

LemonSlice_36

LemonSlice_37

LemonSlice_38

LemonSlice_39

LemonSlice_40

LemonSlice_41

LemonSlice_42

LemonSlice_43

LemonSlice_44

LemonSlice_45

LemonSlice_46
(HTH DNA binding domain)

LemonSlice_47

LemonSlice_48
(lysin A)

LemonSlice_49
(lysin B)

LemonSlice_50

LemonSlice_51

LemonSlice_52
(DNA helicase)

LemonSlice_53

LemonSlice_54

LemonSlice_55

LemonSlice_56

LemonSlice_57

LemonSlice_58

LemonSlice_59
(DNA primase/helicase)

LemonSlice_60

LemonSlice_61
(DNA polymerase I)

LemonSlice_62

LemonSlice_63

LemonSlice_64

LemonSlice_65

LemonSlice_66

LemonSlice_67

LemonSlice_68

LemonSlice_69

LemonSlice_70
(helix-turn-helix DNA binding domain)

LemonSlice_71

LemonSlice_72

LemonSlice_73

LemonSlice_74

LemonSlice_75
(helix-turn-helix DNA binding domain)

LemonSlice_76

LemonSlice_77

LemonSlice_78

LemonSlice_79

LemonSlice_80

LemonSlice_81

LemonSlice_82

LemonSlice_83

LemonSlice_84
(HNH endonuclease)

LemonSlice_85

LemonSlice_86

LemonSlice_87

LemonSlice_88

LemonSlice_89

LemonSlice_90

LemonSlice_91

LemonSlice_92

LemonSlice_93

LemonSlice_94

LemonSlice_95

LemonSlice_96

LemonSlice_97

LemonSlice_98

LemonSlice_99

LemonSlice_100

LemonSlice_101

LemonSlice_102

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF155947
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload