Mycobacterium phage Reprobate
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Detailed Information for Phage Reprobate
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSparky, SparkySAfrica
Found ByLilli Holst
Year Found2010
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates29.8819 S, 30.9877 E Map
Discovery NotesFound at Nelson R. Mandela School of Medicine.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 27, 2012
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage625
Sanger Finishing Reactions16
Genome length (bp)70120
Character of genome endsCircularly Permuted
GC Content68.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB5
Other Cluster Members

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Alis (None)

Azarath (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

Rolex (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

ABU (B1)

Alex (B1)

AltPhacts (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

Cannibal (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Lifestyle (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulumae (B1)

Mag7 (B1)

Maleldil (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Megatron (B1)

Mesh1 (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

RedMaple (B1)

Riggan (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Kamiyu (B3)

Littleengine (B3)

Mortcellus (B3)

NOZO (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

RomaT (B3)

SassBadger (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Plaque NotesPinprick sized clear circular plaques.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes97
Has been Phamerated?Yes
Gene List

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Reprobate_1

Reprobate_2
(Queuine-tRNA ribosyltransferase)

Reprobate_3

Reprobate_4
(monooxygenase)

Reprobate_5
(Terminase, Small Subunit)

Reprobate_6
(Terminase,Large Subunit)

Reprobate_7

Reprobate_8
(RuvC)

Reprobate_9

Reprobate_10
(portal )

Reprobate_11
(capsid morphogenesis protein)

Reprobate_12

Reprobate_13
(Capsid)

Reprobate_14

Reprobate_15

Reprobate_16

Reprobate_17

Reprobate_18

Reprobate_19

Reprobate_20
(Major Tail Subunit)

Reprobate_21

Reprobate_22

Reprobate_23

Reprobate_24

Reprobate_25
(Tail Assembly Chaperone)

Reprobate_26
(Tail Assembly Chaperone)

Reprobate_27
(Tape Measure)

Reprobate_28
(minor tail protein)

Reprobate_29
(minor tail protein)

Reprobate_30
(minor tail protein)

Reprobate_31
(minor tail protein)

Reprobate_32
(minor tail protein)

Reprobate_33

Reprobate_34

Reprobate_35

Reprobate_36

Reprobate_37

Reprobate_38

Reprobate_39

Reprobate_40

Reprobate_41

Reprobate_42

Reprobate_43

Reprobate_44

Reprobate_45

Reprobate_46
(HTH Domain)

Reprobate_47
(Lys A)

Reprobate_48

Reprobate_49
(LysB)

Reprobate_50

Reprobate_51
(Helicase Type III Restriction Subunit)

Reprobate_52

Reprobate_53

Reprobate_54

Reprobate_55
(primase/helicase)

Reprobate_56

Reprobate_57
(DNA Pol 1)

Reprobate_58

Reprobate_59

Reprobate_60

Reprobate_61

Reprobate_62

Reprobate_63

Reprobate_64

Reprobate_65

Reprobate_66

Reprobate_67

Reprobate_68

Reprobate_69
(RNaseE)

Reprobate_70

Reprobate_71

Reprobate_72

Reprobate_73

Reprobate_74

Reprobate_75

Reprobate_76

Reprobate_77

Reprobate_78

Reprobate_79

Reprobate_80

Reprobate_81

Reprobate_82

Reprobate_83

Reprobate_84

Reprobate_85

Reprobate_86

Reprobate_87

Reprobate_88

Reprobate_89

Reprobate_90

Reprobate_91

Reprobate_92

Reprobate_93

Reprobate_94

Reprobate_95

Reprobate_96

Reprobate_97

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF024727
Refseq NumberNC_022064
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload