Mycobacterium phage Heathcliff
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Detailed Information for Phage Heathcliff
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexandra Cathcart
Year Found2011
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.444235 N, 79.95871 W Map
Discovery NotesIn a mulch bed; ~2 centimeters down
At the bottom of a slope
Soil moist; bed littered with trash
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 12, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage130
Sanger Finishing Reactions6
Genome length (bp)68628
Character of genome endsCircularly Permuted
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB3
Other Cluster Members

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Alis (None)

Azarath (None)

BigMau (None)

BlaqueIvy (None)

Cally (None)

Ceci11 (None)

ChrIke (None)

Conway (None)

CptKIRK (None)

DaHileesi (None)

Kerrigan (None)

Loadofcarp (None)

Mage (None)

Meraxes (None)

Muus (None)

Olive00 (None)

Pallavicini (None)

RoseLilly (None)

SeTi1 (None)

Skye (None)

Themis (None)

ABU (B1)

Alex (B1)

Altwerkus (B1)

APE308 (B1)

Apizium (B1)

ArigunDD10 (B1)

Ashraf (B1)

AstrumP28 (B1)

AyJay1824 (B1)

Badfish (B1)

Banjo (B1)

BatteryCK (B1)

BengaliBalla92 (B1)

Beni05 (B1)

BlackStallion (B1)

BRod943 (B1)

Buckeye (B1)

BullsEye (B1)

Cannibal (B1)

Chah (B1)

CharlieGBrown (B1)

CheetO (B1)

Chorkpop (B1)

Chunky (B1)

Cobra (B1)

Coffean (B1)

Colbert (B1)

Cold1 (B1)

Cosmolli16 (B1)

Cowgirl (B1)

Craff (B1)

Crownjwl (B1)

Daffy (B1)

Derpp (B1)

Dingo (B1)

Dione (B1)

Ditto (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

DuffyC (B1)

Durga (B1)

Egahp (B1)

EmmyMG (B1)

EmpTee (B1)

Eremos (B1)

Fang (B1)

Ferhersher (B1)

FluffyNinja (B1)

FriarPreacher (B1)

Fringe (B1)

Frogger (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giddykiddy (B1)

Girly (B1)

Grand2040 (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Harvey (B1)

Held (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Horchata (B1)

ImtiyazSitla (B1)

Inkblot (B1)

IPP (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JangoPhett (B1)

Jbone (B1)

Jillium (B1)

Josephine (B1)

Kailash (B1)

Kat63 (B1)

Keitherie (B1)

Kikipoo (B1)

KingTut (B1)

KingVeVeVe (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

Lasso (B1)

LeeLot (B1)

Legion (B1)

Lego3393 (B1)

Legolas (B1)

LemonSlice (B1)

Longacauda (B1)

LoveandHonor (B1)

LuckyMarjie (B1)

Lulu100 (B1)

Lulumae (B1)

Mag7 (B1)

Malkin71 (B1)

Mana (B1)

Manad (B1)

Maskar (B1)

Megatron (B1)

Mesh1 (B1)

Mikota (B1)

Mistert4 (B1)

MitKao (B1)

Morbach (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Novak (B1)

Numberten (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Oosterbaan (B1)

Orion (B1)

OSmaximus (B1)

Pacos19 (B1)

PDRPv (B1)

Pearsonia (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhenghisKhan (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Pickrel13 (B1)

Piglet (B1)

PinkLady16 (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Pops (B1)

Potter (B1)

Prann (B1)

ProfessorX (B1)

Puhltonio (B1)

PuraVida (B1)

Pyro (B1)

RedMaple (B1)

Rfink25 (B1)

Riggan (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Scoot17C (B1)

Scooterbug (B1)

SDcharge11 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Speckles (B1)

Squid (B1)

Squiggle (B1)

Squirt12 (B1)

Steve27 (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swish (B1)

TallGrassMM (B1)

Thora (B1)

ThreeOh3D2 (B1)

Tigris (B1)

Timmi (B1)

TomBombadil (B1)

Tre (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

Ulysses2016 (B1)

UncleHowie (B1)

Vaishali24 (B1)

Valjean (B1)

Veritas (B1)

Virapocalypse (B1)

Vista (B1)

Vivaldi (B1)

Vivamus (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Wunderlich (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Ypsitucky (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Arbiter (B2)

Ares (B2)

Boyle (B2)

Brownie5 (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Julius (B2)

Kheth (B2)

Laurie (B2)

LizLemon (B2)

Opia (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Rstan (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Bernardo (B3)

ChaChing (B3)

Chandler (B3)

CharlesTrout (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Gadjet (B3)

Gulsi (B3)

Heathcliff (B3)

Kamiyu (B3)

Littleengine (B3)

Mortcellus (B3)

NOZO (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phlyer (B3)

Pipefish (B3)

RomaT (B3)

SassBadger (B3)

Yahalom (B3)

Zollars18 (B3)

Adawi (B4)

AlanGrant (B4)

Bane1 (B4)

Bane2 (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Fortunato (B4)

Frederick (B4)

Halloween (B4)

JAMaL (B4)

LeapingLizzy (B4)

Nigel (B4)

Paliothales9 (B4)

RawrgerThat (B4)

Skruzzles (B4)

Stinger (B4)

TheRipper (B4)

Tvorc (B4)

Vincenzo (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

Donny (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Plaque NotesTwo different size plaques; one pinprick size and one about 1 mm in diameter
No turbidity; plaques very clear and round
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes103
Has been Phamerated?Yes
Gene List

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Heathcliff_1
(ParB)

Heathcliff_2

Heathcliff_3

Heathcliff_4
(PAPS reductase)

Heathcliff_5

Heathcliff_6
(Terminase, large subunit)

Heathcliff_7

Heathcliff_8
(RuvC)

Heathcliff_9

Heathcliff_10

Heathcliff_11

Heathcliff_12
(capsid morphogenesis protein)

Heathcliff_13

Heathcliff_14
(Capsid)

Heathcliff_15

Heathcliff_16

Heathcliff_17

Heathcliff_18

Heathcliff_19

Heathcliff_20
(DNA binding domain)

Heathcliff_21
(Major tail subunit)

Heathcliff_22

Heathcliff_23

Heathcliff_24

Heathcliff_25

Heathcliff_26

Heathcliff_27

Heathcliff_28
(Tapemeasure)

Heathcliff_29
(Minor Tail)

Heathcliff_30
(Minor Tail)

Heathcliff_31
(Minor Tail)

Heathcliff_32
(Minor Tail)

Heathcliff_33
(Minor Tail)

Heathcliff_34

Heathcliff_35

Heathcliff_36

Heathcliff_37

Heathcliff_38

Heathcliff_39

Heathcliff_40

Heathcliff_41

Heathcliff_42
(Hydrolase domain)

Heathcliff_43
(DNA binding domain)

Heathcliff_44
(DNA-binding domain)

Heathcliff_45

Heathcliff_46
(LysA)

Heathcliff_47
(LysB)

Heathcliff_48

Heathcliff_49

Heathcliff_50

Heathcliff_51

Heathcliff_52
(Helicase)

Heathcliff_53

Heathcliff_54

Heathcliff_55

Heathcliff_56
(RepA)

Heathcliff_57

Heathcliff_58
(DNA Pol I)

Heathcliff_59

Heathcliff_60

Heathcliff_61

Heathcliff_62

Heathcliff_63

Heathcliff_64

Heathcliff_65

Heathcliff_66

Heathcliff_67

Heathcliff_68
(PardD DNA binding domain)

Heathcliff_69

Heathcliff_70

Heathcliff_71
(HTH domain)

Heathcliff_72

Heathcliff_73

Heathcliff_74

Heathcliff_75
(Metal binding domain)

Heathcliff_76

Heathcliff_77
(DNA binding domain)

Heathcliff_78

Heathcliff_79

Heathcliff_80
(HNH endonuclease)

Heathcliff_81

Heathcliff_82

Heathcliff_83

Heathcliff_84

Heathcliff_85

Heathcliff_86

Heathcliff_87

Heathcliff_88

Heathcliff_89

Heathcliff_90

Heathcliff_91

Heathcliff_92

Heathcliff_93

Heathcliff_94

Heathcliff_95

Heathcliff_96

Heathcliff_97

Heathcliff_98

Heathcliff_99

Heathcliff_100

Heathcliff_101

Heathcliff_102

Heathcliff_103

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ194584
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload