| Detailed Information for Phage APunk |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Sophia Bieri |
| Year Found | 2020 |
| Location Found | Grand Rapids, MI United States |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.915811 N, 85.644374 W Map |
| Discovery Notes | Collected from the edge of Zuidema Soccer Field, behind the vR wing and parking lot 8. The soil was moist and clumpy. |
| Naming Notes | This phage is named after a song called "A-Punk" by Vampire Weekend. The song is a bop, and my phage is an adorable little bacteria defeating "punk". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1256 |
| Genome length (bp) | 59154 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Cabrini University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were clear and circular, with diameters ranging from 1-2mm across. The edges were clear and defined. When plaques overlapped, the morphology became more difficult to ascertain. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON755186 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
119 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
