Gordonia phage Begonia
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Detailed Information for Phage Begonia
Discovery Information
Isolation HostGordonia terrae 3612
Found ByChelsea Hancock
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.440464 N, 79.955696 W Map
Discovery NotesBegonia was discovered in a patch of dirt next to a bike rack outside of Clapp Hall, the University of Pittsburgh biology building. There was no nearby vegetation, and on the discovery day, the weather was sunny and about 70 degrees.
Naming NotesI have chose the name Begonia after a song by my favorite band, The Grateful Dead.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1049
Genome length (bp)50867
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCGGTGA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionGettysburg College
Annotation StatusIn GenBank
Plaque NotesBegonia produces plaques that range from 0.5 to 1.5 millimeters. Although they range in size, they are all round and completely clear.
MorphotypeSiphoviridae
Number of Genes78
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Begonia_1
(terminase small subunit)

Begonia_2
(terminase large subunit)

Begonia_3
(portal protein)

Begonia_4
(capsid maturation protease)

Begonia_5
(scaffolding protein)

Begonia_6
(major capsid protein)

Begonia_7

Begonia_8
(head-to-tail adaptor)

Begonia_9
(head-to-tail stopper)

Begonia_10

Begonia_11

Begonia_12

Begonia_13
(tail terminator)

Begonia_14
(major tail protein)

Begonia_15
(tail assembly chaperone)

Begonia_16
(tail assembly chaperone)

Begonia_17
(HNH endonuclease)

Begonia_18
(tape measure protein)

Begonia_19
(minor tail protein)

Begonia_20
(minor tail protein)

Begonia_21
(lysin A, L-Ala-D-Glu peptidase domain)

Begonia_22
(lysin A, glycosyl hydrolase domain)

Begonia_23

Begonia_24

Begonia_25

Begonia_26
(lysin B)

Begonia_27

Begonia_28
(minor tail protein)

Begonia_29
(minor tail protein)

Begonia_30

Begonia_31
(minor tail protein)

Begonia_32
(minor tail protein)

Begonia_33

Begonia_34

Begonia_35

Begonia_36

Begonia_37

Begonia_38
(serine integrase)

Begonia_39
(helix-turn-helix DNA binding protein)

Begonia_40
(helix-turn-helix DNA binding protein)

Begonia_41
(immunity repressor)

Begonia_42
(helix-turn-helix DNA binding protein)

Begonia_43

Begonia_44
(antirepressor)

Begonia_45

Begonia_46

Begonia_47

Begonia_48

Begonia_49
(helix-turn-helix DNA binding protein)

Begonia_50

Begonia_51

Begonia_52

Begonia_53

Begonia_54

Begonia_55

Begonia_56
(exonuclease)

Begonia_57
(RecT-like ssDNA binding protein)

Begonia_58

Begonia_59

Begonia_60

Begonia_61

Begonia_62

Begonia_63
(nucleotidyltransferase)

Begonia_64
(helix-turn-helix DNA binding protein)

Begonia_65

Begonia_66

Begonia_67

Begonia_68

Begonia_69

Begonia_70

Begonia_71

Begonia_72

Begonia_73
(RuvC-like resolvase)

Begonia_74

Begonia_75
(helix-turn-helix DNA binding protein)

Begonia_76

Begonia_77

Begonia_78
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK878896
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.6 x 10^10 pfu / mL
Pitt Freezer Box# 56
Pitt Freezer Box Grid# H7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload