| Detailed Information for Phage Begonia |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Chelsea Hancock |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.440464 N, 79.955696 W Map |
| Discovery Notes | Begonia was discovered in a patch of dirt next to a bike rack outside of Clapp Hall, the University of Pittsburgh biology building. There was no nearby vegetation, and on the discovery day, the weather was sunny and about 70 degrees. |
| Naming Notes | I have chose the name Begonia after a song by my favorite band, The Grateful Dead. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1049 |
| Genome length (bp) | 50867 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCGGTGA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | Begonia produces plaques that range from 0.5 to 1.5 millimeters. Although they range in size, they are all round and completely clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK878896 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.6 x 10^10 pfu / mL |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
H7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
