Mycobacterium phage Butters
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Detailed Information for Phage Butters
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLena Ma
Year Found2011
Location FoundPhiladelphia, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.06067 N, 75.046608 W Map
Discovery NotesPhage was discovered from a soil sample in Philadelphia at 25 degrees Celcius. The temperature is sunny and 78% humidity. The soil sample collected was dry and obtained from soil 3cm deep. The soil was also near growing plants where there was only sunlight (no shade).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 29, 2012
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage280
Genome length (bp)41491
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCCCTCG
GC Content65.8%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesAfter 24 hours of incubation, the plaque sizes are all 0.5mm in diameter and turbid. After 48 hours of incubation the plaques grew to be two different sizes. The first size is 0.5mm-1.0mm in diameter and turbid. The second size is 2.0mm-3.0mm in diameter and turbid. Even with the two different sizes the plaques yield the same phage. After incubation for longer than 48 hours, the plaques are all 3.0mm in diameter and bullseye. The bullseye effect is only shown after incubation of longer than 48 hours. Titers were performed from one of the 0.5mm-1.0mm plaques and from one of the 2.0mm-3.0mm plaques and both titers yielded the same morphology as discussed above, thus concluding that even with the two sized plaques, the phage is the same.
The phage is a siphoviridae with an icosahedral head but looks almost spherical on the EM picture. It has a 3:1 tail:head ratio and has horizontal banding on its tail. There is also a small ball on the end of the tail.
Number of Genes66
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKC576783
Refseq NumberNC_021061
Published in PaperHatfull et al 2013, Genome Announcements: Complete genome sequences of 63 mycobacteriophages
Archiving Info
Archiving status Archived
SEA Designator 2011LEHIbuttersLM
Pitt Freezer Box# 25
Pitt Freezer Box Grid# 56
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload