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Mycobacterium phage Duplicity
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Detailed Information for Phage Duplicity
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlex Jennings
Year Found2014
Location FoundArkadelphia, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.126111 N, 93.052222 W Map
Naming NotesWhen trying to isolate a single phage it looked to be like two phages in my plates and it wasn't until the 6th Titer Assay that we came to the conclusion that is ONE phage creating these plaques. So I have named it Duplicity because that means deceitful or two.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1210
Genome length (bp)42938
Character of genome ends3' Sticky Overhang
Overhang Length14 bases
Overhang SequenceCCCGCCGCAATGGG
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusBeing Annotated (Expected completion by 11/30/2021)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5 x 10 ^13
Pitt Freezer Box# 8
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
EM PictureDownload