Mycobacterium phage Melville
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Detailed Information for Phage Melville
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOlivia Neilly and Conner Dunathan
Year Found2023
Location FoundBoulder, CO United States
Finding InstitutionUniversity of Colorado Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature2°C
GPS Coordinates40.0 N, 105.0 W Map
Discovery NotesTaken from soil next to a building after rainy/snowy conditions. A large amount of soil was collected that was near the sidewalk.
Naming NotesWe named our named Melville to ensure that we followed the phage naming rules.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1418
Genome length (bp)43252
Character of genome ends3' Sticky Overhang
Overhang Length14 bases
GC Content66.3%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Colorado Boulder
Annotation StatusAwaiting_QC
Plaque NotesPlaques are approximately 3 mm in diameter and have halo shapes around a majority of plaques. Morphology seen on the plaque assay supports the idea that temperate phages are present due to their cloudy appearance.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 178
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload