Mycobacterium phage Chewbacca
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Detailed Information for Phage Chewbacca
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegan Kays and Rodney Tan
Year Found2017
Location FoundLas Vegas, NV USA
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.233013 N, 115.302754 W Map
Discovery NotesCollected from private home at Latitude 36.109452 Longitude -115.143600 at a depth of 6". The soil was moist, sandy, and filled with debris. It was collected from a semi-shady location under a 9 year old pomegranate tree that had been fertilized with organic homemade compost 3 months prior. Ambient temperature was 94 degrees Fahrenheit
Naming NotesBecause Chewbacca is awesome and has a giant crossbow.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 12, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3156
Genome length (bp)43575
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesLysogenic, especially in large concentrations. Only enters the lytic stage in diluted concentrations.
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesA lysogen that is consistently .4 mm in diameter, extremely turbid with a clearly defined halo on the exterior of plaque, with or without a bulls-eye in center.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Chewbacca_1
(terminase, small subunit)

Chewbacca_2
(terminase, large subunit)

Chewbacca_3

Chewbacca_4
(portal protein)

Chewbacca_5
(capsid maturation protease)

Chewbacca_6
(major capsid protein)

Chewbacca_7

Chewbacca_8
(head-tail connector complex protein)

Chewbacca_9
(head-tail connector complex protein)

Chewbacca_10
(head-tail connector complex protein)

Chewbacca_11
(head-tail connector complex protein)

Chewbacca_12
(head-tail connector complex protein)

Chewbacca_13
(major tail protein)

Chewbacca_14
(tail assembly chaperone)

Chewbacca_15
(tail assembly chaperone)

Chewbacca_16
(tape measure protein)

Chewbacca_17
(minor tail protein)

Chewbacca_18
(minor tail protein)

Chewbacca_19
(minor tail protein)

Chewbacca_20
(minor tail protein)

Chewbacca_21

Chewbacca_22

Chewbacca_23

Chewbacca_24

Chewbacca_25

Chewbacca_26

Chewbacca_27

Chewbacca_28
(lysin A)

Chewbacca_29
(holin)

Chewbacca_30
(membrane protein)

Chewbacca_31

Chewbacca_32
(toxin in toxin/antitoxin system, HicA-like)

Chewbacca_33
(toxin in toxin/antitoxin system, HicB-like)

Chewbacca_34

Chewbacca_35

Chewbacca_36

Chewbacca_37
(integrase)

Chewbacca_38
(immunity repressor)

Chewbacca_39
(excise)

Chewbacca_40

Chewbacca_41

Chewbacca_42

Chewbacca_43

Chewbacca_44

Chewbacca_45

Chewbacca_46
(WhiB family transcription factor)

Chewbacca_47
(WhiB family transcription factor)

Chewbacca_48
(WhiB family transcription factor)

Chewbacca_49
(exonuclease)

Chewbacca_50
(RecT-like ssDNA binding protein)

Chewbacca_51
(RusA-like resolvase (endonuclease))

Chewbacca_52
(NrdH glutaredoxin)

Chewbacca_53

Chewbacca_54

Chewbacca_55

Chewbacca_56

Chewbacca_57

Chewbacca_58

Chewbacca_59
(mycobacteriophage mobile element 1 (MPME 1))

Chewbacca_60

Chewbacca_61

Chewbacca_62

Chewbacca_63

Chewbacca_64
(HNH endonuclease)

Chewbacca_65

Chewbacca_66

Chewbacca_67
(helix-turn-telix DNA binding domain)

Chewbacca_68

Chewbacca_69

Chewbacca_70

Chewbacca_71
(helix-turn-telix DNA binding domain)

Chewbacca_72

Chewbacca_73

Chewbacca_74
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH926055
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.33 x 10^-10
Pitt Freezer Box# 52
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload