| Detailed Information for Phage Raymond7 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rayna Smilowitz |
| Year Found | 2016 |
| Location Found | Hewlett, NY USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.637 N, 73.685 W Map |
| Discovery Notes | -retrieved January 14, 2016 at approximately 3:00pm -Temperature: 38 degrees Fahrenheit- 3.33 degrees Celsius - 4 inches: approximately 10 cm - area was moist, rural, rose to trees, 40 ft from a building, not new any foot traffic, flat ground |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 23, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1481 |
| Genome length (bp) | 42382 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCCTTGG |
| GC Content | 66.0% |
| Sequencing Notes | The sequencing reads indicated that approximately 60% of this population has a 4386-base deletion from position 24006 to 28391, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | There were some problems in calculating the lysate. I went through a bunch of spot tests and 10 plate/ single plate flooding processes. They were all relatively low in calculation but my DNA calculation was a good number. I stuck with my initial 10 plate lysate which had a concentration of 1.0x10^8 mL. |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity Fulbright Gex Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Xeno Xerxes |
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were relatively ranged in sizes from 0.8cm to 0.25cm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRaymond7_1 Raymond7_2 Raymond7_3 Raymond7_4 Raymond7_5 Raymond7_6 Raymond7_7 Raymond7_8 Raymond7_9 Raymond7_10 Raymond7_11 Raymond7_12 Raymond7_13 Raymond7_14 Raymond7_15 Raymond7_16 Raymond7_17 Raymond7_18 Raymond7_19 Raymond7_20 Raymond7_21 Raymond7_22 Raymond7_23 Raymond7_24 Raymond7_25 Raymond7_26 Raymond7_27 Raymond7_28 Raymond7_29 Raymond7_30 Raymond7_31 Raymond7_32 Raymond7_33 Raymond7_34 Raymond7_35 Raymond7_36 Raymond7_37 Raymond7_38 Raymond7_39 Raymond7_40 Raymond7_41 Raymond7_42 Raymond7_43 Raymond7_44 Raymond7_45 Raymond7_46 Raymond7_47 Raymond7_48 Raymond7_49 Raymond7_50 Raymond7_51 Raymond7_52 Raymond7_53 Raymond7_54 Raymond7_55 Raymond7_56 Raymond7_57 Raymond7_58 Raymond7_59 Raymond7_60 Raymond7_61 Raymond7_62 Raymond7_63 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT498048 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.0x10^8 |
| Date of SEA Lysate Titering | Apr 19, 2016 |
| Pitt Freezer Box# | 35 |
| Pitt Freezer Box Grid# | C7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
