Mycobacterium phage Rebel
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Detailed Information for Phage Rebel
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJulia Reisner
Year Found2016
Location FoundMerrick, NY USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates40.654922 N, 73.540769 W Map
Discovery NotesSoil sample collected on January 17th, 2016 from Merrick New York. The ground was frozen so we used a shovel to take soil from underneath a bush. The soil was taken from the top of the ground.

Gp30 aligns with other Cluster N immunity repressors, but is truncated. For example, the Butters immunity repressor is 123 amino acids long, but Rebel Gp30 is predicted to be 96 amino acids in length with amino acids missing from the C terminal end. The function of gp30 is therefore called as "immunity repressor, truncated". Note that no integrase is annotated in the genome. it is unlikely that Rebel would form a stable lysogen.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 26, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1504
Genome length (bp)40580
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCCTTGG
GC Content66.5%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesClear, very small plaques (1mm). Are present after 24 hours of incubation, but are extremely hard to see. Much more visible after 48 hours of incubation.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT498042
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.33E10
Date of SEA Lysate Titering Apr 14, 2016
Pitt Freezer Box# 35
Pitt Freezer Box Grid# C8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload