Mycobacterium phage Phloss
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Detailed Information for Phage Phloss
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySummer Apostalo, Gavin Meyer
Year Found2020
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.4675 N, 94.725 W Map
Discovery NotesOn Tuesday, August 25, 2020 a soil sample of approximately 12 milliliters was collected on a cloudless, sunny morning with a temperature of 75°.It was located approximately 1 meter from the roots of a group of green hedge bushes which lie between two small trees. The area of collection was well shaded by both the hedge bushes and the adjacent trees.The collection site prior to sample extraction was covered by a thin layer of dead leaves and small sticks. After brushing the debris away from the dig site, exposing the soil below, a plastic spoon was used to dig a hole approximately 1.5 inch in diameter and 1.5 inch deep, from which the soil sample was collected.
Naming NotesThe name of this phage came from a play on words for 'plaque'. Plaques can also be found in mouths and "floss" or "phloss" in our case, can be used to clean teeth of any plaques.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 22, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2005
Genome length (bp)43107
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.3%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP297540
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 118
Pitt Freezer Box Grid# D2
Available Files
EM PictureDownload
GenBank File for PhameratorDownload