Mycobacterium phage Journey
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Detailed Information for Phage Journey
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesmok226@lehigh.edu
Found ByMokhinur Kodirova
Year Found2023
Location FoundBethleham, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature3°C
GPS Coordinates40.603027 N, 75.80618 W Map
Discovery NotesSoil comes from university dorm - House 89, it was collected at 3:16pm, and was collected approximately 5cm below the surface. Soil contained mostly organic matter, and a little bit of sand with small twigs in it.
Naming NotesI named my phage Journey because the journey that it took me to find my phage was meaningful, fun and the best part of everything I did.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1762
Genome length (bp)41986
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusBeing Annotated (Expected completion by 5/1/2024)
Plaque NotesMy plaque has turbid lysis with a diameter of 1mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.33 *10^10
Pitt Freezer Box# 186
Pitt Freezer Box Grid# D11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload