| Detailed Information for Phage Kevin1 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kevin Zhang |
| Year Found | 2016 |
| Location Found | Naugatuck, CT USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.490695 N, 73.056109 W Map |
| Discovery Notes | My soil sample was found in my backyard at a temperature of 0 degrees celsius in the winter. It was found next to a tree about 2 inches deep into the ground. The soil sample was also initially very solid and hard because of the cold temperature. |
| Naming Notes | The name of my phage, Kevin, is named after his awesome founder Kevin Zhang. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 17, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 6219 |
| Genome length (bp) | 41988 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCCCTCG |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are all similar in size and in shape. They are all circular and about 2 mm in diameter. Plaques on the plates are pretty consistent in terms of morphology and size. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524500 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.12 x 10^12 |
| Date of SEA Lysate Titering |
Mar 29, 2016 |
| Pitt Freezer Box# |
35 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |