Mycobacterium phage Nenae
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Detailed Information for Phage Nenae
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexa Alhout
Year Found2016
Location FoundFairfield, NJ USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.883739 N, 74.305992 W Map
Discovery NotesThe soil was collected in approximately 35 degrees weather. The soil was taken from a mint garden.
Naming NotesI chose the name Nenae (Nau-naa)because that is how you say mint in arabic.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 17, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3222
Genome length (bp)42597
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCCTTGG
GC Content66.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesThe phage is a siphoviridae. The head is approximately 70 nm in length and width. The tail length is 200 nm.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524520
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.3x10^10 pfu/ml
Date of SEA Lysate Titering Mar 31, 2016
Pitt Freezer Box# 35
Pitt Freezer Box Grid# C2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload