| Detailed Information for Phage Charlie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sanjeev Chalissery, Neil Mistry |
| Year Found | 2009 |
| Location Found | Richmond, VA USA |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.544958 N, 77.454041 W Map |
| Discovery Notes | 75 deg F, collected under a cherry tree in mulched soil, 2 cm |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 22, 2011 |
| Genome length (bp) | 43036 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques aren't completely round. ~1.5mm diameter, clear with turbid edge. |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JN256079 |
| Refseq Number | NC_023729 |
| Published in Paper | Hatfull et al 2012, Journal of Virology: Complete genome sequences of 138 mycobacteriophages |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2009VACUCharlieSCNM |
| SEA Lysate Titer |
3.60e11 |
| Date of SEA Lysate Titering |
Nov 15, 2009 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
