| Detailed Information for Phage Cubone | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Triniti Antony and Amber Viar |
| Year Found | 2025 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.21 N, 80.45 W Map |
| Discovery Notes | The phage was found outside a residential area under a tree. It was found at 72°F in a dry, soiled environment. |
| Naming Notes | The name was chosen in honor of the Pokémon character. It was cute like our phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 206 |
| Genome length (bp) | 44310 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Cubone Duplicity EGUnicorn Fulbright Gex Hanako Impisi Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon Spinach SpongeBob Tapioca Tortoise12 Xeno Xerxes |
| Annotating Institution | Virginia Tech |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaque is small, circular, and has a hazy edge. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewCubone_1 Cubone_2 Cubone_3 Cubone_4 Cubone_5 Cubone_6 Cubone_7 Cubone_8 Cubone_9 Cubone_10 Cubone_11 Cubone_12 Cubone_13 Cubone_14 Cubone_15 Cubone_16 Cubone_17 Cubone_18 Cubone_19 Cubone_20 Cubone_21 Cubone_22 Cubone_23 Cubone_24 Cubone_25 Cubone_26 Cubone_27 Cubone_28 Cubone_29 Cubone_30 Cubone_31 Cubone_32 Cubone_33 Cubone_34 Cubone_35 Cubone_36 — tRNA Cubone_37 Cubone_38 Cubone_39 Cubone_40 Cubone_41 Cubone_42 Cubone_43 Cubone_44 Cubone_45 Cubone_46 Cubone_47 Cubone_48 Cubone_49 Cubone_50 Cubone_51 Cubone_52 Cubone_53 Cubone_54 Cubone_55 Cubone_56 Cubone_57 Cubone_58 Cubone_59 Cubone_60 Cubone_61 Cubone_62 Cubone_63 Cubone_64 Cubone_65 Cubone_66 Cubone_67 Cubone_68 Cubone_69 Cubone_70 Cubone_71 Cubone_72 Cubone_73 Cubone_74 Cubone_75 Cubone_76 Cubone_77 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
