Mycobacterium phage Duplicity
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Detailed Information for Phage Duplicity
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlex Jennings
Year Found2014
Location FoundArkadelphia, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.126111 N, 93.052222 W Map
Naming NotesWhen trying to isolate a single phage it looked to be like two phages in my plates and it wasn't until the 6th Titer Assay that we came to the conclusion that is ONE phage creating these plaques. So I have named it Duplicity because that means deceitful or two.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1210
Genome length (bp)42938
Character of genome ends3' Sticky Overhang
Overhang Length14 bases
Overhang SequenceCCCGCCGCAATGGG
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes70
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Duplicity_1

Duplicity_2
(terminase large subunit)

Duplicity_3

Duplicity_4
(portal protein)

Duplicity_5
(capsid maturation protease)

Duplicity_6
(major capsid protein)

Duplicity_7

Duplicity_8
(head-to-tail adaptor)

Duplicity_9
(head-to-tail stopper)

Duplicity_10

Duplicity_11

Duplicity_12
(tail terminator)

Duplicity_13
(major tail protein)

Duplicity_14
(tail assembly chaperone)

Duplicity_15
(tail assembly chaperone)

Duplicity_16
(tape measure protein)

Duplicity_17
(minor tail protein)

Duplicity_18
(minor tail protein)

Duplicity_19
(minor tail protein)

Duplicity_20
(minor tail protein)

Duplicity_21

Duplicity_22
(minor tail protein)

Duplicity_23

Duplicity_24

Duplicity_25

Duplicity_26

Duplicity_27

Duplicity_28
(lysin A)

Duplicity_29
(holin)

Duplicity_30

Duplicity_31

Duplicity_32

Duplicity_33

Duplicity_34

Duplicity_35

Duplicity_36
(tyrosine integrase)

Duplicity_37
(immunity repressor)

Duplicity_38
(excise)

Duplicity_39

Duplicity_40

Duplicity_41

Duplicity_42

Duplicity_43

Duplicity_44

Duplicity_45
(WhiB family transcription factor)

Duplicity_46

Duplicity_47

Duplicity_48
(RecT-like DNA pairing protein)

Duplicity_49
(holliday junction resolvase)

Duplicity_50
(NrdH-like glutaredoxin)

Duplicity_51

Duplicity_52

Duplicity_53

Duplicity_54

Duplicity_55

Duplicity_56

Duplicity_57

Duplicity_58

Duplicity_59
(HNH endonuclease)

Duplicity_60

Duplicity_61

Duplicity_62

Duplicity_63

Duplicity_64

Duplicity_65

Duplicity_66
(helix-turn-helix DNA binding protein)

Duplicity_67

Duplicity_68

Duplicity_69

Duplicity_70
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOL742561
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5 x 10 ^13
Pitt Freezer Box# 8
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload