| Detailed Information for Phage Guacamole |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Rebecca Rohac |
| Year Found | 2015 |
| Location Found | Pittsburgh , PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.445396 N, 79.957995 W Map |
| Discovery Notes | -Weather conditions during collection: overcast but still humid, 85 degrees fahrenheit
-Weather conditions 24hrs prior to collection: less sun and more clouds, highs in the upper 80s and lows in the 70s
- Time of collection: 3:00pm, sun was at its peak
-Soil Conditions: warm, damp, area, with lots of leaves present
- Soil was transported in backpack for about 5-6 hours before being properly stored.
Found in lawn across from the Chevron Science Center, in the back lawn of the Space Research Coordination Center |
| Naming Notes | The name for this phage was inspired by Chipotle Mexican Grille's delicious guacamole. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 809 |
| Genome length (bp) | 49894 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | most likely reproduces through the lytic cycle due to its plaque morphology |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small circles with a clear center |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU963259 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
H10 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
