| Detailed Information for Phage Journey |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | mok226@lehigh.edu |
| Found By | Mokhinur Kodirova |
| Year Found | 2023 |
| Location Found | Bethleham, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 3°C |
| GPS Coordinates | 40.603027 N, 75.80618 W Map |
| Discovery Notes | Soil comes from university dorm - House 89, it was collected at 3:16pm, and was collected approximately 5cm below the surface. Soil contained mostly organic matter, and a little bit of sand with small twigs in it. |
| Naming Notes | I named my phage Journey because the journey that it took me to find my phage was meaningful, fun and the best part of everything I did. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1762 |
| Genome length (bp) | 41986 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaque has turbid lysis with a diameter of 1mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978786 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.33 *10^10 |
| Pitt Freezer Box# |
186 |
| Pitt Freezer Box Grid# |
D11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
