Mycobacterium phage Kevin1
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Detailed Information for Phage Kevin1
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKevin Zhang
Year Found2016
Location FoundNaugatuck, CT USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.490695 N, 73.056109 W Map
Discovery NotesMy soil sample was found in my backyard at a temperature of 0 degrees celsius in the winter. It was found next to a tree about 2 inches deep into the ground. The soil sample was also initially very solid and hard because of the cold temperature.
Naming NotesThe name of my phage, Kevin, is named after his awesome founder Kevin Zhang.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 17, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage6219
Genome length (bp)41988
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCCCTCG
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesPlaques are all similar in size and in shape. They are all circular and about 2 mm in diameter. Plaques on the plates are pretty consistent in terms of morphology and size.
MorphotypeSiphoviridae
Number of Genes69
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524500
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.12 x 10^12
Date of SEA Lysate Titering Mar 29, 2016
Pitt Freezer Box# 35
Pitt Freezer Box Grid# B11
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload