Gordonia phage Malachai
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Detailed Information for Phage Malachai
Discovery Information
Isolation HostGordonia terrae 3612
Found ByJ. Bremigen, K. LeCompte, A. Le, A. Levitsky
Year Found2020
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.4451 N, 79.9564 W Map
Discovery NotesTaken from Soldiers’ and Sailors’ lawn in Pittsburgh. Soil is dry with few pieces of grass/leaves.

Discovered by: Jarrett Bremigen, Kaitlyn LeCompte, Angelina Levitsky, Angela Le
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 27, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4518
Genome length (bp)50867
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCGGTGA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesMalachai shows mesas when in very high concentrations, so it is therefore a temperate phage.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPhage Malachi has a halo morphology and its size averages between 1 mm and 1.5 mm.
Number of Genes77
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Malachai_1
(terminase small subunit)

Malachai_2
(terminase large subunit)

Malachai_3
(portal protein)

Malachai_4
(capsid maturation protease)

Malachai_5
(scaffolding protein)

Malachai_6
(major capsid protein)

Malachai_7

Malachai_8
(head-to-tail adaptor)

Malachai_9
(head-to-tail stopper)

Malachai_10

Malachai_11

Malachai_12

Malachai_13
(tail terminator)

Malachai_14
(major tail protein)

Malachai_15
(tail assembly chaperone)

Malachai_16
(tail assembly chaperone)

Malachai_17
(HNH endonuclease)

Malachai_18
(tape measure protein)

Malachai_19
(minor tail protein)

Malachai_20
(minor tail protein)

Malachai_21
(lysin A, L-Ala-D-Glu peptidase domain)

Malachai_22
(lysin A, glycosyl hydrolase domain)

Malachai_23
(holin)

Malachai_24

Malachai_25

Malachai_26
(lysin B)

Malachai_27

Malachai_28
(minor tail protein)

Malachai_29
(minor tail protein)

Malachai_30

Malachai_31
(minor tail protein)

Malachai_32

Malachai_33

Malachai_34

Malachai_35

Malachai_36

Malachai_37

Malachai_38
(serine integrase)

Malachai_39
(helix-turn-helix DNA binding domain protein)

Malachai_40
(helix-turn-helix DNA binding domain protein)

Malachai_41
(immunity repressor)

Malachai_42
(helix-turn-helix DNA binding domain protein)

Malachai_43

Malachai_44
(antirepressor)

Malachai_45

Malachai_46

Malachai_47

Malachai_48

Malachai_49
(helix-turn-helix DNA binding domain protein)

Malachai_50

Malachai_51

Malachai_52

Malachai_53

Malachai_54

Malachai_55

Malachai_56
(RecE-like exonuclease)

Malachai_57
(RecT-like ssDNA binding protein)

Malachai_58

Malachai_59

Malachai_60

Malachai_61

Malachai_62

Malachai_63
(nucleotidyl transferase)

Malachai_64
(ASC-1 transcription coactivator)

Malachai_65
(DnaJ-like chaperonin)

Malachai_66

Malachai_67

Malachai_68

Malachai_69

Malachai_70

Malachai_71

Malachai_72

Malachai_73
(RuvC-like resolvase)

Malachai_74

Malachai_75
(helix-turn-helix DNA binding domain protein)

Malachai_76

Malachai_77
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON724011
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 125
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload