| Detailed Information for Phage Malachai |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | J. Bremigen, K. LeCompte, A. Le, A. Levitsky |
| Year Found | 2020 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4451 N, 79.9564 W Map |
| Discovery Notes | Taken from Soldiers’ and Sailors’ lawn in Pittsburgh. Soil is dry with few pieces of grass/leaves.
Discovered by: Jarrett Bremigen, Kaitlyn LeCompte, Angelina Levitsky, Angela Le |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4518 |
| Genome length (bp) | 50867 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCGGTGA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Malachai shows mesas when in very high concentrations, so it is therefore a temperate phage. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Phage Malachi has a halo morphology and its size averages between 1 mm and 1.5 mm. |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON724011 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
125 |
| Pitt Freezer Box Grid# |
G5 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
