| Detailed Information for Phage Parmesanjohn |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ryan Seth and Sarah Misiukiewicz |
| Year Found | 2017 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.604448 N, 75.374991 W Map |
| Discovery Notes | Found outside of the Dravo building at Lehigh University |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 25, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 201 |
| Genome length (bp) | 43700 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.3% |
| Sequencing Notes | phage was sequenced as part of a DOGEMS sample from Lehigh 2017
at position 18963 bp approximately 30% of reads contain an A instead of consensus T |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques have diameter of 2 mm and appear 24-48 hours after plating. Center of each plaque is clear and becomes more turbid as you travel further from the center. |
| Morphotype | Siphoviridae |
| Number of Genes | 72 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524515 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.67E10 pfu/ml |
| Date of SEA Lysate Titering |
Mar 19, 2017 |
| Pitt Freezer Box# |
51 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
