| Detailed Information for Phage Phloss | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Summer Apostalo, Gavin Meyer |
| Year Found | 2020 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.4675 N, 94.725 W Map |
| Discovery Notes | On Tuesday, August 25, 2020 a soil sample of approximately 12 milliliters was collected on a cloudless, sunny morning with a temperature of 75°.It was located approximately 1 meter from the roots of a group of green hedge bushes which lie between two small trees. The area of collection was well shaded by both the hedge bushes and the adjacent trees.The collection site prior to sample extraction was covered by a thin layer of dead leaves and small sticks. After brushing the debris away from the dig site, exposing the soil below, a plastic spoon was used to dig a hole approximately 1.5 inch in diameter and 1.5 inch deep, from which the soil sample was collected. |
| Naming Notes | The name of this phage came from a play on words for 'plaque'. Plaques can also be found in mouths and "floss" or "phloss" in our case, can be used to clean teeth of any plaques. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2005 |
| Genome length (bp) | 43107 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity Fulbright Gex Hanako Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Xeno Xerxes |
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPhloss_1 Phloss_2 Phloss_3 Phloss_4 Phloss_5 Phloss_6 Phloss_7 Phloss_8 Phloss_9 Phloss_10 Phloss_11 Phloss_12 Phloss_13 Phloss_14 Phloss_15 Phloss_16 Phloss_17 Phloss_18 Phloss_19 Phloss_20 Phloss_21 Phloss_22 Phloss_23 Phloss_24 Phloss_25 Phloss_26 Phloss_27 Phloss_28 Phloss_29 Phloss_30 Phloss_31 Phloss_32 Phloss_33 Phloss_34 Phloss_35 Phloss_36 Phloss_37 Phloss_38 Phloss_39 Phloss_40 Phloss_41 Phloss_42 Phloss_43 Phloss_44 Phloss_45 Phloss_46 Phloss_47 Phloss_48 Phloss_49 Phloss_50 Phloss_51 Phloss_52 Phloss_53 Phloss_54 Phloss_55 Phloss_56 Phloss_57 Phloss_58 Phloss_59 Phloss_60 Phloss_61 Phloss_62 Phloss_63 Phloss_64 Phloss_65 Phloss_66 Phloss_67 Phloss_68 Phloss_69 Phloss_70 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP297540 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 118 |
| Pitt Freezer Box Grid# | D2 |
| Available Files | |
| EM Picture | Download |
| GenBank File for Phamerator | Download |