| Detailed Information for Phage Rebel | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julia Reisner |
| Year Found | 2016 |
| Location Found | Merrick, NY USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.654922 N, 73.540769 W Map |
| Discovery Notes | Soil sample collected on January 17th, 2016 from Merrick New York. The ground was frozen so we used a shovel to take soil from underneath a bush. The soil was taken from the top of the ground. Gp30 aligns with other Cluster N immunity repressors, but is truncated. For example, the Butters immunity repressor is 123 amino acids long, but Rebel Gp30 is predicted to be 96 amino acids in length with amino acids missing from the C terminal end. The function of gp30 is therefore called as "immunity repressor, truncated". Note that no integrase is annotated in the genome. it is unlikely that Rebel would form a stable lysogen. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 26, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1504 |
| Genome length (bp) | 40580 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCCTTGG |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity Fulbright Gex Hanako Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Xeno Xerxes |
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, very small plaques (1mm). Are present after 24 hours of incubation, but are extremely hard to see. Much more visible after 48 hours of incubation. |
| Morphotype | Siphoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRebel_1 Rebel_2 Rebel_3 Rebel_4 Rebel_5 Rebel_6 Rebel_7 Rebel_8 Rebel_9 Rebel_10 Rebel_11 Rebel_12 Rebel_13 Rebel_14 Rebel_15 Rebel_16 Rebel_17 Rebel_18 Rebel_19 Rebel_20 Rebel_21 Rebel_22 Rebel_23 Rebel_24 Rebel_25 Rebel_26 Rebel_27 Rebel_28 Rebel_29 Rebel_30 Rebel_31 Rebel_32 Rebel_33 Rebel_34 Rebel_35 Rebel_36 Rebel_37 Rebel_38 Rebel_39 Rebel_40 Rebel_41 Rebel_42 Rebel_43 Rebel_44 Rebel_45 Rebel_46 Rebel_47 Rebel_48 Rebel_49 Rebel_50 Rebel_51 Rebel_52 Rebel_53 Rebel_54 Rebel_55 Rebel_56 Rebel_57 Rebel_58 Rebel_59 Rebel_60 Rebel_61 Rebel_62 Rebel_63 Rebel_64 Rebel_65 Rebel_66 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT498042 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.33E10 |
| Date of SEA Lysate Titering | Apr 14, 2016 |
| Pitt Freezer Box# | 35 |
| Pitt Freezer Box Grid# | C8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
