| Detailed Information for Phage SkinnyPete |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kristen Wade |
| Year Found | 2012 |
| Location Found | Richmond, VA USA |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.325703 N, 77.205717 W Map |
| Discovery Notes | Found in a rain water run-off collection pool at the VCU Rice Center. About 6 inches under the surface, plants/algae in the water. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 15, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 380 |
| Sanger Finishing Reactions | 13 |
| Genome length (bp) | 43478 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCCTTGG |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Mostly clear, slightly turbid around edges of plaque. Large and small sizes. Large: 2.5-3mm diameter, small:0.5-1mm diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 67 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU935729 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
65 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
