| Detailed Information for Phage Tortoise12 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julia Gregory and Amanda White |
| Year Found | 2024 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.245 N, 80.424 W Map |
| Discovery Notes | Found outside in planted mulch in low foot-traffic area. |
| Naming Notes | The apartment where phage was found is called the Hub in Blacksburg (shortned to BBurg). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 102 |
| Genome length (bp) | 42689 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were small and circular with varying sizes. Edges were clean. |
| Morphotype | Siphoviridae |
| Number of Genes | 72 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915844 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
211 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
