| Detailed Information for Phage Tortoise12 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julia Gregory and Amanda White |
| Year Found | 2024 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.245 N, 80.424 W Map |
| Discovery Notes | Found outside in planted mulch in low foot-traffic area. |
| Naming Notes | The apartment where phage was found is called the Hub in Blacksburg (shortned to BBurg). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 102 |
| Genome length (bp) | 42689 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity EGUnicorn Fulbright Gex Hanako Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Tortoise12 Xeno Xerxes |
| Annotating Institution | Virginia Tech |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Plaques were small and circular with varying sizes. Edges were clean. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTortoise12_1 Tortoise12_2 Tortoise12_3 Tortoise12_4 Tortoise12_5 Tortoise12_6 Tortoise12_7 Tortoise12_8 Tortoise12_9 Tortoise12_10 Tortoise12_11 Tortoise12_12 Tortoise12_13 Tortoise12_14 Tortoise12_15 Tortoise12_16 Tortoise12_17 Tortoise12_18 Tortoise12_19 Tortoise12_20 Tortoise12_21 Tortoise12_22 Tortoise12_23 Tortoise12_24 Tortoise12_25 Tortoise12_26 Tortoise12_27 Tortoise12_28 Tortoise12_29 Tortoise12_30 Tortoise12_31 Tortoise12_32 Tortoise12_33 Tortoise12_34 Tortoise12_35 Tortoise12_36 — tRNA Tortoise12_37 Tortoise12_38 Tortoise12_39 Tortoise12_40 Tortoise12_41 Tortoise12_42 Tortoise12_43 Tortoise12_44 Tortoise12_45 Tortoise12_46 Tortoise12_47 Tortoise12_48 Tortoise12_49 Tortoise12_50 Tortoise12_51 Tortoise12_52 Tortoise12_53 Tortoise12_54 Tortoise12_55 Tortoise12_56 Tortoise12_57 Tortoise12_58 Tortoise12_59 Tortoise12_60 Tortoise12_61 Tortoise12_62 Tortoise12_63 Tortoise12_64 Tortoise12_65 Tortoise12_66 Tortoise12_67 Tortoise12_68 Tortoise12_69 Tortoise12_70 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
