| Detailed Information for Phage Chewbacca |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Kays and Rodney Tan |
| Year Found | 2017 |
| Location Found | Las Vegas, NV USA |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.233013 N, 115.302754 W Map |
| Discovery Notes | Collected from private home at Latitude 36.109452 Longitude -115.143600 at a depth of 6". The soil was moist, sandy, and filled with debris. It was collected from a semi-shady location under a 9 year old pomegranate tree that had been fertilized with organic homemade compost 3 months prior. Ambient temperature was 94 degrees Fahrenheit |
| Naming Notes | Because Chewbacca is awesome and has a giant crossbow. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3156 |
| Genome length (bp) | 43575 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Lysogenic, especially in large concentrations. Only enters the lytic stage in diluted concentrations. |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | A lysogen that is consistently .4 mm in diameter, extremely turbid with a clearly defined halo on the exterior of plaque, with or without a bulls-eye in center. |
| Morphotype | Siphoviridae |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH926055 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.33 x 10^-10 |
| Pitt Freezer Box# |
52 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
