| Detailed Information for Phage Chewbacca | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Kays and Rodney Tan |
| Year Found | 2017 |
| Location Found | Las Vegas, NV USA |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.233013 N, 115.302754 W Map |
| Discovery Notes | Collected from private home at Latitude 36.109452 Longitude -115.143600 at a depth of 6". The soil was moist, sandy, and filled with debris. It was collected from a semi-shady location under a 9 year old pomegranate tree that had been fertilized with organic homemade compost 3 months prior. Ambient temperature was 94 degrees Fahrenheit |
| Naming Notes | Because Chewbacca is awesome and has a giant crossbow. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3156 |
| Genome length (bp) | 43575 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | CCCGCCGCAATGG |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | N |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Lysogenic, especially in large concentrations. Only enters the lytic stage in diluted concentrations. |
| Other Cluster Members |
Click to ViewAggie Andies BabeRuth Bosection6 Butters Carcharodon Charlie Chewbacca Duplicity EGUnicorn Fulbright Gex Hanako Jamie19 Journey Kevin1 Magsby Melville MichelleMyBell Nenae Panchino Parmesanjohn PhancyPhin Philonius Phloss Phrann Pipsqueaks Purgamenstris Raymond7 Rebel Redi Rubeelu Schnauzer Scitech ShrimpFriedEgg Shweta Silvafighter Silvy SkinnyPete Smurph Snekmaggedon SpongeBob Tapioca Tortoise12 Xeno Xerxes |
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | A lysogen that is consistently .4 mm in diameter, extremely turbid with a clearly defined halo on the exterior of plaque, with or without a bulls-eye in center. |
| Morphotype | Siphoviridae |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewChewbacca_1 Chewbacca_2 Chewbacca_3 Chewbacca_4 Chewbacca_5 Chewbacca_6 Chewbacca_7 Chewbacca_8 Chewbacca_9 Chewbacca_10 Chewbacca_11 Chewbacca_12 Chewbacca_13 Chewbacca_14 Chewbacca_15 Chewbacca_16 Chewbacca_17 Chewbacca_18 Chewbacca_19 Chewbacca_20 Chewbacca_21 Chewbacca_22 Chewbacca_23 Chewbacca_24 Chewbacca_25 Chewbacca_26 Chewbacca_27 Chewbacca_28 Chewbacca_29 Chewbacca_30 Chewbacca_31 Chewbacca_32 Chewbacca_33 Chewbacca_34 Chewbacca_35 Chewbacca_36 Chewbacca_37 Chewbacca_38 Chewbacca_39 Chewbacca_40 Chewbacca_41 Chewbacca_42 Chewbacca_43 Chewbacca_44 Chewbacca_45 Chewbacca_46 Chewbacca_47 Chewbacca_48 Chewbacca_49 Chewbacca_50 Chewbacca_51 Chewbacca_52 Chewbacca_53 Chewbacca_54 Chewbacca_55 Chewbacca_56 Chewbacca_57 Chewbacca_58 Chewbacca_59 Chewbacca_60 Chewbacca_61 Chewbacca_62 Chewbacca_63 Chewbacca_64 Chewbacca_65 Chewbacca_66 Chewbacca_67 Chewbacca_68 Chewbacca_69 Chewbacca_70 Chewbacca_71 Chewbacca_72 Chewbacca_73 Chewbacca_74 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH926055 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.33 x 10^-10 |
| Pitt Freezer Box# | 52 |
| Pitt Freezer Box Grid# | C11 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
