| Detailed Information for Phage Grayscar | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Scarlett Mayfield |
| Year Found | 2025 |
| Location Found | Saint Louis, MO United States |
| Finding Institution | St. Louis Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 38.552003 N, 90.28303 W Map |
| Discovery Notes | Found in backyard. Moist soil. Sample taken from about 5-6 inches down. |
| Naming Notes | I picked the name Grayscar because it is a combination of my brother and I's name and something my dad has always used for gaming and things like that. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 16, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Read Length | 300 bp |
| Approximate Shotgun Coverage | 1526 |
| Genome length (bp) | 17453 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno DirtPie Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 ELee24 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery Grayscar HarperAnne HeadMave Hernandez44 Horacetta Hulk IzzyPants24 Jahseh JaimeB Jannah Jella Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Madebyisha Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Myram Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus OdetoJoy Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trackstar Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba Zahlia |
| Annotating Institution | St. Louis Community College |
| Annotation Status | Being Annotated (Expected completion by 4/16/2026) |
| Plaque Notes | The plaques have been small to medium sized. |
| Number of Genes | 26 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGrayscar_1 Grayscar_2 Grayscar_3 Grayscar_4 Grayscar_5 Grayscar_6 Grayscar_7 Grayscar_8 Grayscar_9 Grayscar_10 Grayscar_11 Grayscar_12 Grayscar_13 — tRNA Grayscar_14 Grayscar_15 Grayscar_16 Grayscar_17 Grayscar_18 Grayscar_19 Grayscar_20 Grayscar_21 Grayscar_22 Grayscar_23 Grayscar_24 Grayscar_25 Grayscar_26 Grayscar_27 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |