| Detailed Information for Phage Majesty | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Queen Lee-Mayes |
| Year Found | 2019 |
| Location Found | Petersburg, VA united states |
| Finding Institution | Virginia State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.2369 N, 77.4208 W Map |
| Discovery Notes | It was 69 degrees outside on cloudy and foggy day. The sample was just beneath the top layer of the soil. |
| Naming Notes | "Majesty" has always been an essential name in my life. When I started working with my phage, I had a lot of errors. These errors caused me to redo protocols. Working with "Majesty" has been a humbling experience. This lesson taught me to be patient while working with a dignified phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2965 |
| Genome length (bp) | 17444 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Azizam Aztec Baine BarBear Belthelas BinkBonk BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Chatham Cheesecake Ciel Clownery Concrete Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Erin Gardevoir Gillker GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix PuffyCat Quaker Rhogar Rhysand Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yasuo YertPhresh Yubaba |
| Annotating Institution | Virginia State University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques were arranged randomly. At times my serial dilutions had very confusing concentrated phage samples. I had trouble making webbed plates, mainly because I had so much bacteria that they eventually "ate" each other. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMajesty_1 Majesty_2 Majesty_3 Majesty_4 Majesty_5 Majesty_6 Majesty_7 Majesty_8 Majesty_9 Majesty_10 Majesty_11 Majesty_12 Majesty_13 Majesty_14 Majesty_15 Majesty_16 Majesty_17 Majesty_18 Majesty_19 Majesty_20 Majesty_21 Majesty_22 Majesty_23 Majesty_24 Majesty_25 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT776807 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 106 |
| Pitt Freezer Box Grid# | G9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |