Microbacterium phage Majesty
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Detailed Information for Phage Majesty
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByQueen Lee-Mayes
Year Found2019
Location FoundPetersburg, VA united states
Finding InstitutionVirginia State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.2369 N, 77.4208 W Map
Discovery NotesIt was 69 degrees outside on cloudy and foggy day. The sample was just beneath the top layer of the soil.
Naming Notes"Majesty" has always been an essential name in my life. When I started working with my phage, I had a lot of errors. These errors caused me to redo protocols. Working with "Majesty" has been a humbling experience. This lesson taught me to be patient while working with a dignified phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2965
Genome length (bp)17444
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionVirginia State University
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesMy plaques were arranged randomly. At times my serial dilutions had very confusing concentrated phage samples. I had trouble making webbed plates, mainly because I had so much bacteria that they eventually "ate" each other.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload