| Detailed Information for Phage Rhysand | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Allison Smith |
| Year Found | 2018 |
| Location Found | Rock Hill, SC USA |
| Finding Institution | Winthrop University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.939064 N, 81.028002 W Map |
| Discovery Notes | Rhysand was discovered from a soil sample retrieved on September 11, 2018, from the base of an oak tree on the outskirts of DInkin's Lot at Winthrop University. It was collected at a depth of approximately 1 inch and the ambient temperature was 24.2°C. |
| Naming Notes | Named after my favorite character from the Court of Thorns and Roses series written by Sara J. Maas. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 29, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4518 |
| Genome length (bp) | 17453 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Plaques showed no signs of a lysogenic period. Plaques appeared to be completely lytic. |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Winthrop University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques formed quickly, beginning as small plaques before rapidly combining with all nearby plaques, forming medium sized plaques. The plaques appeared to be completely lytic. They grew at a moderate plaque density. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRhysand_1 Rhysand_2 Rhysand_3 Rhysand_4 Rhysand_5 Rhysand_6 Rhysand_7 Rhysand_8 Rhysand_9 Rhysand_10 Rhysand_11 Rhysand_12 Rhysand_13 Rhysand_14 Rhysand_15 Rhysand_16 Rhysand_17 Rhysand_18 Rhysand_19 Rhysand_20 Rhysand_21 Rhysand_22 Rhysand_23 Rhysand_24 Rhysand_25 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK620897 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 81 |
| Pitt Freezer Box Grid# | B1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
