Microbacterium phage MelBee03
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Detailed Information for Phage MelBee03
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByMadelyn Brookins
Year Found2021
Location FoundHudson, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates44.984533 N, 92.7545 W Map
Discovery NotesFound in Hudson,WI from a compost pile next to West side of a garage, in a alley way.
Adopted from Karen Klyczek
Naming NotesI chose the name MelBee03. MelBee03, comes from a singer that is a judge on American Idol. Her name is actually spelled Mel B, but I thought it would be fun to add bee to it. And 03 rhymes with bee and 03 is my birth date.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 23, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage99
Genome length (bp)17441
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusBeing Annotated (Expected completion by 9/23/2022)
Plaque NotesThey are all the same size, the plates are not cloudy and can see the plaques just fine. None of my plaques had halos, until lysate titer.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 140
Pitt Freezer Box Grid# C9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload