Microbacterium phage Belthelas
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Detailed Information for Phage Belthelas
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByHope Kirby, Hannah Wilson
Year Found2018
Location FoundOxford, OH USA
Finding InstitutionMiami University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates39.514 N, 84.7261 W Map
Discovery NotesIt was found from lichen near the base of a tree about thirty feet away from a creek. It was near undeveloped hiking trails. The tree was about 2 feet thick, it was humid, and there were fungi and decomposing leaves near the bottom as well but on the ground.
Naming NotesBelthelas= Belthor + Athelas
Belthor: Elvish for Killer
Athelas: powerful herb in Lord of the Rings used by Aragorn to heal Eowyn and Faramir of the dark powers found in pure evil. It was thought to be useless until the king came and called for it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3320
Genome length (bp)17502
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionMiami University
Annotation StatusIn GenBank
Plaque Notes- Started out as large halo, but shrunk with time until the turbid halo was only about a twentieth of the plaque. There did not appear to be any elevation.
- Most started out relatively large, but with time also the plaques became smaller as more bacteria was killed
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK801730
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.86x10^11
Pitt Freezer Box# 74
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload