| Detailed Information for Phage Eightball |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alexis Rivera-Manning |
| Year Found | 2020 |
| Location Found | Chester, PA United States |
| Finding Institution | Neumann University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.841972 N, 75.389932 W Map |
| Discovery Notes | Found at 9:24 pm in 71 F weather. |
| Naming Notes | The phage grew at a fast rate since the beginning days of lab. In pool, your goal is to get your either stripped or solids balls in each pocket before the other person. Once in the pockets you can now hit the final ball or "eightball" in the pocket to win. Like pool my phages development was quickier than others and im waiting for the end game to come to hit my final ball in the pocket. This gave me the idea of calling the phage eightball. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5973 |
| Genome length (bp) | 17439 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Neumann University |
| Annotation Status | In GenBank |
| Plaque Notes | Large round clear plaques about 2 mm in size. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK040783 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.88 x 10^10 |
| Pitt Freezer Box# |
120 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
