| Detailed Information for Phage JoBros | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Cassidy H, Emily D, Taylor K, Ivy S |
| Year Found | 2019 |
| Location Found | South Portland, ME United States |
| Finding Institution | Southern Maine Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 43.646504 N, 70.227752 W Map |
| Discovery Notes | During the continuous process of plaque assays to isolate our phage, there was on and off development of phage. Some plaque assays provided us with phage, some had cross-contamination, which was distinguished by the development of other forms of bacteria. For plaque assays #4 and #5, our 10^-4 lysate plate had developed phage that was best suitable for us to further isolate. |
| Naming Notes | The Jonas Bothers Band released a new album the day we began our phage hunt. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4487 |
| Genome length (bp) | 17363 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Azizam Aztec Baine BarBear Belthelas BinkBonk BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Chatham Cheesecake Ciel Clownery Concrete Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Erin Gardevoir Gillker GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix PuffyCat Quaker Rhogar Rhysand Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yasuo YertPhresh Yubaba |
| Annotating Institution | Allegany College of Maryland |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewJoBros_1 JoBros_2 JoBros_3 JoBros_4 JoBros_5 JoBros_6 JoBros_7 JoBros_8 JoBros_9 JoBros_10 JoBros_11 JoBros_12 JoBros_13 JoBros_14 JoBros_15 JoBros_16 JoBros_17 JoBros_18 JoBros_19 JoBros_20 JoBros_21 JoBros_22 JoBros_23 JoBros_24 JoBros_25 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW055907 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |