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Microbacterium phage JoBros
JoBros EM Picture
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Detailed Information for Phage JoBros
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByCassidy H, Emily D, Taylor K, Ivy S
Year Found2019
Location FoundSouth Portland, ME United States
Finding InstitutionSouthern Maine Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates43.646504 N, 70.227752 W Map
Discovery NotesDuring the continuous process of plaque assays to isolate our phage, there was on and off development of phage. Some plaque assays provided us with phage, some had cross-contamination, which was distinguished by the development of other forms of bacteria. For plaque assays #4 and #5, our 10^-4 lysate plate had developed phage that was best suitable for us to further isolate.
Naming NotesThe Jonas Bothers Band released a new album the day we began our phage hunt.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4487
Genome length (bp)17363
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionAllegany College of Maryland
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload