Microbacterium phage Livingwater
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Detailed Information for Phage Livingwater
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByMatthew Crawford
Year Found2019
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.0271 N, 86.5099 W Map
Naming NotesThis bacteriophage was found in the Gasper River near a Christian Retreat Center. It would be fitting to use the term living water since it is a Christian retreat area, although viruses are not living.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2308
Genome length (bp)17450
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWestern Kentucky University
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesThe plaque morphology is bullseye. The plaques have a clear center with a turbid halo.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.5x10^10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload