Detailed Information for Phage Livingwater |
Discovery Information |
Isolation Host | Microbacterium foliorum NRRL B-24224 |
Found By | Matthew Crawford |
Year Found | 2019 |
Location Found | Bowling Green, KY United States |
Finding Institution | Western Kentucky University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 37.0271 N, 86.5099 W Map |
Naming Notes | This bacteriophage was found in the Gasper River near a Christian Retreat Center. It would be fitting to use the term living water since it is a Christian retreat area, although viruses are not living. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 29, 2020 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 2308 |
Genome length (bp) | 17450 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CCCGCCCCA |
GC Content | 68.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | EE |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Western Kentucky University |
Annotation Status | In GenBank |
Plaque Notes | The plaque morphology is bullseye. The plaques have a clear center with a turbid halo. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT498040 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
2.5x10^10 |
Pitt Freezer Box# |
100 |
Pitt Freezer Box Grid# |
D10 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |