Microbacterium phage Alucard
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Detailed Information for Phage Alucard
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByJakob O'Neal
Year Found2021
Location FoundSouth Portland, ME United States
Finding InstitutionSouthern Maine Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates43.647224 N, 70.227765 W Map
Discovery NotesThe sample the phage was found in was taken from Southern Maine Community College's community garden. The sample was moist, dark soil taken from a tomato patch.
Naming NotesI had Halloween on the brain when I was thinking of a name for the phage. While it isn't a perfect comparison, how phages survive and replicate remind me of vampires. "Alucard" is "Dracula" spelled backwards.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5143
Genome length (bp)17363
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionSouthern Maine Community College
Annotation StatusIn GenBank
Plaque NotesThe plaques are 3 mm in diameter on average. The plaques appear to have present but not starkly apparent halos, indicating that the phages are temperate.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR521072
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 183
Pitt Freezer Box Grid# E10
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload