Microbacterium phage Wolfpack
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Detailed Information for Phage Wolfpack
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByNoah Gansluckner
Year Found2019
Location FoundPlum City, WI United States of America
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates44.67 N, 92.27 W Map
Discovery NotesThe soil was collected at 4:00 p.m. It was collected in a former barnyard that hadn't been tended to in 5 years. The soil was moist and loose with some grass roots in it. The soil was collected at 30mm below the surface. The air temperature was 57 degrees F. It was collected on a misty day, with a light drizzle.
Naming NotesWolfpack was the nickname of my high school athletic mascot. My roommate Nic Forster recommended it to me when we were sitting in our room and I asked him.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 8, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage145
Genome length (bp)17455
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusBeing Annotated (Expected completion by 11/8/2020)
Plaque NotesI originally had two different types of phages when performing my assays, one small and one large. I decided to isolate and continue with the larger plaque phage. It leaves a large, clear plaque with only a tiny dot of bacteria left in the middle. There is no halo and the plaque is about 2mm in diameter and width . The plaques were virtually all uniform, with all of them being almost all clear and very large.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 102
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload