| Detailed Information for Phage Wolfpack | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Noah Gansluckner |
| Year Found | 2019 |
| Location Found | Plum City, WI United States of America |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.67 N, 92.27 W Map |
| Discovery Notes | The soil was collected at 4:00 p.m. It was collected in a former barnyard that hadn't been tended to in 5 years. The soil was moist and loose with some grass roots in it. The soil was collected at 30mm below the surface. The air temperature was 57 degrees F. It was collected on a misty day, with a light drizzle. |
| Naming Notes | Wolfpack was the nickname of my high school athletic mascot. My roommate Nic Forster recommended it to me when we were sitting in our room and I asked him. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 8, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 145 |
| Genome length (bp) | 17455 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAnseraureola Aries55 Azizam Aztec BarBear Belthelas BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 BurtonThePup Cheesecake Ciel Concrete Danno Dongwon Efeko Eightball Gardevoir HarperAnne Hernandez44 Hulk Jahseh Jannah Jerky JoBros Josuke JRok Kaijohn KayPaulus LaviMo Leafy Livingwater Lola20 Lunatic Luxx Majesty ManAs McShie Miaurora Minima MrGreen Naby Namsahir NeumannU Nobel Noelani Octopus Otwor Owens PaoPu Pondwater PoRanda Quaker Rhysand Sara Scamander Scrunchy SeaWolves Sippinontea Slentz TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Vanisius VitulaEligans Wolfpack Yasuo YertPhresh Yubaba |
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | I originally had two different types of phages when performing my assays, one small and one large. I decided to isolate and continue with the larger plaque phage. It leaves a large, clear plaque with only a tiny dot of bacteria left in the middle. There is no halo and the plaque is about 2mm in diameter and width . The plaques were virtually all uniform, with all of them being almost all clear and very large. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewWolfpack_1 Wolfpack_2 Wolfpack_3 Wolfpack_4 Wolfpack_5 Wolfpack_6 Wolfpack_7 Wolfpack_8 Wolfpack_9 Wolfpack_10 Wolfpack_11 Wolfpack_12 Wolfpack_13 Wolfpack_14 Wolfpack_15 Wolfpack_16 Wolfpack_17 Wolfpack_18 Wolfpack_19 Wolfpack_20 Wolfpack_21 Wolfpack_22 Wolfpack_23 Wolfpack_24 Wolfpack_25 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW507127 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 102 |
| Pitt Freezer Box Grid# | H12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
