| Detailed Information for Phage Wolfpack | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Noah Gansluckner |
| Year Found | 2019 |
| Location Found | Plum City, WI United States of America |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.67 N, 92.27 W Map |
| Discovery Notes | The soil was collected at 4:00 p.m. It was collected in a former barnyard that hadn't been tended to in 5 years. The soil was moist and loose with some grass roots in it. The soil was collected at 30mm below the surface. The air temperature was 57 degrees F. It was collected on a misty day, with a light drizzle. |
| Naming Notes | Wolfpack was the nickname of my high school athletic mascot. My roommate Nic Forster recommended it to me when we were sitting in our room and I asked him. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 8, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 145 |
| Genome length (bp) | 17455 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAries55 Azizam Aztec Belthelas BonaeVitae BoomRoasted Bradley2 Bri160 Bullzi2019 BurtonThePup Cheesecake Ciel Concrete Danno Dongwon Efeko HarperAnne Hernandez44 Hulk Jahseh Jerky JoBros JRok Kaijohn KayPaulus LaviMo Livingwater Lunatic Luxx Majesty McShie Miaurora Minima Naby Namsahir NeumannU Nobel Noelani Octopus Otwor Owens PaoPu PoRanda Quaker Rhysand Scamander Scrunchy SeaWolves Sippinontea Slentz TeddyBoy TimoTea TinyTruffula Trireme Vanisius VitulaEligans Wolfpack YertPhresh |
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | Being Annotated (Expected completion by 11/8/2020) |
| Plaque Notes | I originally had two different types of phages when performing my assays, one small and one large. I decided to isolate and continue with the larger plaque phage. It leaves a large, clear plaque with only a tiny dot of bacteria left in the middle. There is no halo and the plaque is about 2mm in diameter and width . The plaques were virtually all uniform, with all of them being almost all clear and very large. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewWolfpack_Draft_1 Wolfpack_Draft_2 Wolfpack_Draft_3 Wolfpack_Draft_4 Wolfpack_Draft_5 Wolfpack_Draft_6 Wolfpack_Draft_7 Wolfpack_Draft_8 Wolfpack_Draft_9 Wolfpack_Draft_10 Wolfpack_Draft_11 Wolfpack_Draft_12 Wolfpack_Draft_13 Wolfpack_Draft_14 Wolfpack_Draft_15 Wolfpack_Draft_16 Wolfpack_Draft_17 Wolfpack_Draft_18 Wolfpack_Draft_19 Wolfpack_Draft_20 Wolfpack_Draft_21 Wolfpack_Draft_22 Wolfpack_Draft_23 Wolfpack_Draft_24 Wolfpack_Draft_25 Wolfpack_Draft_26 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 102 |
| Pitt Freezer Box Grid# | H12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
