| Detailed Information for Phage PoRanda |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Miranda Tiffany |
| Year Found | 2019 |
| Location Found | Cullowhee, NC United States |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.310556 N, 83.187222 W Map |
| Discovery Notes | Was found by stream on Western Carolina University's campus. The enrichment sample was a mixture of dirt and sand. The sample was taken from the surface of a riverbank. |
| Naming Notes | My friend and I were joking about a ring I was wearing that day. He said I was the "Panda Warrior." After continuing the joke, he decided he was going to call me PoRanda. He is in the Phage Hunters program with me, so it seemed fitting that my nickname in lab should be my phage's name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4592 |
| Genome length (bp) | 17510 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 69.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques have clear centers and a foggy halo around the outside. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT498065 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1x10^10 PFU/mL |
| Pitt Freezer Box# |
105 |
| Pitt Freezer Box Grid# |
F3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
