Recently Added Phages

VRao Melu Khadka Kalonda Efron

Recently Modified Phages

Leah Luke Frisbee FrenchToast Foogazy

Recently Finished Phages

Sephiroth (DU) Gole (None) Mandlovu (F1) Hlubikazi (F1) Mova (F1)
Microbacterium phage SeaWolves
SeaWolves EM Picture
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage SeaWolves
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Former namesPhinders
Found ByChelsea Lachance, Adeline McDonald, Abigail Ackor
Year Found2019
Location FoundSouth Portland, ME United States
Finding InstitutionSouthern Maine Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates43.6459 N, 70.2276 W Map
Discovery NotesWe looked in the back of our Biology & Science Center building. We looked for dirt that had possible phage. We stayed near the building in hopes of finding phage that had never been discovered.
Naming NotesWe were searching for Phage, which was a new term and lesson in our Biology class. We originally had the name PhagePhinders but the website doesn't allow the word "phage".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5744
Genome length (bp)17362
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionSouthern Maine Community College
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesWhen creating plaques, we made sure there was no contamination when moving from each level with dilutions. We needed to infect the culture tubes before creating the plaque assays and be careful when pouring the petri dish to cover and not have bubbles creating any confusion. After being incubated, we found phage after several tests without any lysate.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives