Microbacterium phage SeaWolves
SeaWolves EM Picture
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Detailed Information for Phage SeaWolves
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Former namesPhinders
Found ByChelsea Lachance, Adeline McDonald, Abigail Ackor
Year Found2019
Location FoundSouth Portland, ME United States
Finding InstitutionSouthern Maine Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates43.6459 N, 70.2276 W Map
Discovery NotesWe looked in the back of our Biology & Science Center building. We looked for dirt that had possible phage. We stayed near the building in hopes of finding phage that had never been discovered.
Naming NotesWe were searching for Phage, which was a new term and lesson in our Biology class. We originally had the name PhagePhinders but the website doesn't allow the word "phage".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5744
Genome length (bp)17362
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionColumbia State Community College
Annotation StatusIn GenBank
Plaque NotesWhen creating plaques, we made sure there was no contamination when moving from each level with dilutions. We needed to infect the culture tubes before creating the plaque assays and be careful when pouring the petri dish to cover and not have bubbles creating any confusion. After being incubated, we found phage after several tests without any lysate.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ709210
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
GenBank File for PhameratorDownload