| Detailed Information for Phage Minima |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Dathan Stone |
| Year Found | 2017 |
| Location Found | Guilford, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.3172 N, 72.7209 W Map |
| Discovery Notes | The sample was found in the morning, 70F and the soil was a mix of dry and wet. About 10mm was dug down, and grass was pulled to mix in with the soil sample. Since microbacterium foliorum was discovered in the phylosphere of grasses, I wanted to mix some of the grass into the soil. |
| Naming Notes | "Minima" is Latin for "tiny". Minima produces 1mm plaques that look like archery targets. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 9882 |
| Genome length (bp) | 17362 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Southern Connecticut State University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is 1mm, and has a small clear center, with a noticeable fuzzy ring around it. This resembles a bullseye target and is a temperate phage. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651181 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.7 x 10^10 |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
B5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
