| Detailed Information for Phage JRok |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Allison Lecitshon & Gracious Anithanam |
| Year Found | 2019 |
| Location Found | Dupont, PA United States |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.323289 N, 75.741829 W Map |
| Discovery Notes | Found in grandparents driveway, with lots of trees and cars around. Was around 6 feet from the road. Sample was collected by a clean shovel to avoid cross contamination. |
| Naming Notes | We decided to name our phage after the nickname we gave our favorite biology professor. His name is Jared Rokowski, but everyone called him JRok |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 151 |
| Genome length (bp) | 17382 |
| Character of genome ends | 3' Sticky Overhang |
| GC Content | 68.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | 4-5 millimeters, bullseye center. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT639647 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.04*10^9 |
| Pitt Freezer Box# |
98 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
