| Detailed Information for Phage JoBros |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Cassidy H, Emily D, Taylor K, Ivy S |
| Year Found | 2019 |
| Location Found | South Portland, ME United States |
| Finding Institution | Southern Maine Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 43.646504 N, 70.227752 W Map |
| Discovery Notes | During the continuous process of plaque assays to isolate our phage, there was on and off development of phage. Some plaque assays provided us with phage, some had cross-contamination, which was distinguished by the development of other forms of bacteria. For plaque assays #4 and #5, our 10^-4 lysate plate had developed phage that was best suitable for us to further isolate. |
| Naming Notes | The Jonas Bothers Band released a new album the day we began our phage hunt. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 4487 |
| Genome length (bp) | 17363 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Allegany College of Maryland |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW055907 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |